Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12512 | 5' | -50.7 | NC_003345.1 | + | 66187 | 0.66 | 0.952185 |
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Target: 5'- cGGGACUGaaUCAUcGUcgggaacaAGCUcuccAUCAGCGAGa -3' miRNA: 3'- -UCCUGAC--AGUA-UA--------UCGA----UGGUCGCUC- -5' |
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| 12512 | 5' | -50.7 | NC_003345.1 | + | 26008 | 0.66 | 0.942767 |
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Target: 5'- cGGGACUGgagaggAUGGCUucuCCAGCa-- -3' miRNA: 3'- -UCCUGACagua--UAUCGAu--GGUCGcuc -5' |
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| 12512 | 5' | -50.7 | NC_003345.1 | + | 36922 | 0.68 | 0.878654 |
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Target: 5'- cGGGCUGUUc---AGCcguacaGCCAGCGAGa -3' miRNA: 3'- uCCUGACAGuauaUCGa-----UGGUCGCUC- -5' |
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| 12512 | 5' | -50.7 | NC_003345.1 | + | 785 | 0.7 | 0.80848 |
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Target: 5'- gGGGACg--CAUAUGGCUACgcccccggaaAGCGGGa -3' miRNA: 3'- -UCCUGacaGUAUAUCGAUGg---------UCGCUC- -5' |
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| 12512 | 5' | -50.7 | NC_003345.1 | + | 7028 | 1.09 | 0.00395 |
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Target: 5'- aAGGACUGUCAUAUAGCUACCAGCGAGu -3' miRNA: 3'- -UCCUGACAGUAUAUCGAUGGUCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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