Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12513 | 3' | -50.6 | NC_003345.1 | + | 16500 | 0.66 | 0.962218 |
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Target: 5'- -cUCCGGuGGuCGAUGGugagaaugaugcuGAGUUGCUGa -3' miRNA: 3'- gaAGGUCuCC-GUUGCC-------------UUCAACGAUg -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 31912 | 0.66 | 0.950124 |
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Target: 5'- --cCCugAGGGGCGAuuCGGggGcUGUUACa -3' miRNA: 3'- gaaGG--UCUCCGUU--GCCuuCaACGAUG- -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 75738 | 0.66 | 0.950124 |
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Target: 5'- --aCCGcGAGcGCGACGGAacagcucgccgcAGUUGCgGCg -3' miRNA: 3'- gaaGGU-CUC-CGUUGCCU------------UCAACGaUG- -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 77319 | 0.66 | 0.940426 |
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Target: 5'- uUUCCAGucgGGGUcggAugGGGAGgggucgGCUACu -3' miRNA: 3'- gAAGGUC---UCCG---UugCCUUCaa----CGAUG- -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 26566 | 0.67 | 0.935151 |
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Target: 5'- -gUCCGGGGugguaGCGGCGGAGGUggGUcgGCa -3' miRNA: 3'- gaAGGUCUC-----CGUUGCCUUCAa-CGa-UG- -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 54260 | 0.67 | 0.923737 |
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Target: 5'- gUUCCAGcGGgGACGGAGGUUcagACu -3' miRNA: 3'- gAAGGUCuCCgUUGCCUUCAAcgaUG- -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 29673 | 0.68 | 0.904461 |
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Target: 5'- --gCUGGAGGCGGCGGggGUUu---- -3' miRNA: 3'- gaaGGUCUCCGUUGCCuuCAAcgaug -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 25645 | 0.69 | 0.864291 |
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Target: 5'- -cUCCGGuaucuaccgaggucAGGCGugGGAAGUUgaGCUGa -3' miRNA: 3'- gaAGGUC--------------UCCGUugCCUUCAA--CGAUg -5' |
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| 12513 | 3' | -50.6 | NC_003345.1 | + | 7780 | 1.11 | 0.002829 |
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Target: 5'- gCUUCCAGAGGCAACGGAAGUUGCUACa -3' miRNA: 3'- -GAAGGUCUCCGUUGCCUUCAACGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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