Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12514 | 3' | -57.3 | NC_003345.1 | + | 66030 | 0.66 | 0.723383 |
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Target: 5'- -cCCGCuaCGggaaUCCgACCUCCCCGAUg- -3' miRNA: 3'- gcGGCG--GCaa--AGG-UGGAGGGGCUAac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 3716 | 0.66 | 0.713152 |
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Target: 5'- gGCCGCUccg-CgACCUUCCCGAa-- -3' miRNA: 3'- gCGGCGGcaaaGgUGGAGGGGCUaac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 14421 | 0.66 | 0.702848 |
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Target: 5'- gGCCGUCG--UCCACUU-CCCGAa-- -3' miRNA: 3'- gCGGCGGCaaAGGUGGAgGGGCUaac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 37800 | 0.66 | 0.698708 |
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Target: 5'- uGCCGCCGUcaugCCACacccaaugucgcCCCCGAc-- -3' miRNA: 3'- gCGGCGGCAaa--GGUGga----------GGGGCUaac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 20027 | 0.67 | 0.682059 |
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Target: 5'- gGCUGacuUGgagUUCgACCUCCCCGAUg- -3' miRNA: 3'- gCGGCg--GCa--AAGgUGGAGGGGCUAac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 30874 | 0.68 | 0.597896 |
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Target: 5'- -aUCGCC-UUUCCACacaUCCCCGaAUUGa -3' miRNA: 3'- gcGGCGGcAAAGGUGg--AGGGGC-UAAC- -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 73425 | 0.69 | 0.505328 |
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Target: 5'- cCGCCGCCGggUUCUGCCgaCUCGAUg- -3' miRNA: 3'- -GCGGCGGCa-AAGGUGGagGGGCUAac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 16822 | 0.71 | 0.401597 |
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Target: 5'- gGCgGUCGUg-CCACgUUCCCCGAUUGu -3' miRNA: 3'- gCGgCGGCAaaGGUG-GAGGGGCUAAC- -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 50690 | 0.72 | 0.367362 |
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Target: 5'- -cCCGCCGUUUCCACCgCCgCCGu--- -3' miRNA: 3'- gcGGCGGCAAAGGUGGaGG-GGCuaac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 936 | 0.72 | 0.351014 |
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Target: 5'- cCGCC-CCGgUUCCGUCUCCCCGGUc- -3' miRNA: 3'- -GCGGcGGCaAAGGUGGAGGGGCUAac -5' |
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| 12514 | 3' | -57.3 | NC_003345.1 | + | 9098 | 1.09 | 0.001144 |
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Target: 5'- gCGCCGCCGUUUCCACCUCCCCGAUUGa -3' miRNA: 3'- -GCGGCGGCAAAGGUGGAGGGGCUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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