Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12517 | 5' | -50.9 | NC_003345.1 | + | 73278 | 0.66 | 0.964093 |
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Target: 5'- uGUCCuucgcGGAGAAGuuGAagcCCGUGUAg -3' miRNA: 3'- gCAGG-----UCUCUUCggCUga-GGUAUAUg -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 17941 | 0.66 | 0.947578 |
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Target: 5'- cCGUCCAcGGAAGaCCGgcgagaagGCUCCGUGc-- -3' miRNA: 3'- -GCAGGUcUCUUC-GGC--------UGAGGUAUaug -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 70763 | 0.66 | 0.941781 |
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Target: 5'- uCGUCCAGAGGgcGGUCGGagugaccgagCAUAUACg -3' miRNA: 3'- -GCAGGUCUCU--UCGGCUgag-------GUAUAUG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 32178 | 0.67 | 0.920769 |
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Target: 5'- aGUCguGGGAAGCCGACguugacUACa -3' miRNA: 3'- gCAGguCUCUUCGGCUGagguauAUG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 36566 | 0.68 | 0.886981 |
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Target: 5'- cCGUCCGGuAGAAGuCCGuCUCCugAUAg -3' miRNA: 3'- -GCAGGUC-UCUUC-GGCuGAGGuaUAUg -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 31966 | 0.68 | 0.879411 |
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Target: 5'- aG-CCGG-GAAGuCCGACUCCGU-UACc -3' miRNA: 3'- gCaGGUCuCUUC-GGCUGAGGUAuAUG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 46847 | 0.69 | 0.871583 |
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Target: 5'- -cUCCGGGGAguagaagugGGCCGACagcucucgcaCCGUGUACg -3' miRNA: 3'- gcAGGUCUCU---------UCGGCUGa---------GGUAUAUG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 63819 | 0.69 | 0.871583 |
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Target: 5'- uCGUUCuuuGAGAcGaCCGACUCCAgcgGCg -3' miRNA: 3'- -GCAGGu--CUCUuC-GGCUGAGGUauaUG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 14773 | 0.69 | 0.855174 |
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Target: 5'- cCGUCgCGGAGAAGCaCGacacGCUCCAcGUcGCa -3' miRNA: 3'- -GCAG-GUCUCUUCG-GC----UGAGGUaUA-UG- -5' |
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| 12517 | 5' | -50.9 | NC_003345.1 | + | 12048 | 1.1 | 0.003511 |
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Target: 5'- aCGUCCAGAGAAGCCGACUCCAUAUACc -3' miRNA: 3'- -GCAGGUCUCUUCGGCUGAGGUAUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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