Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12518 | 5' | -52.7 | NC_003345.1 | + | 42115 | 0.66 | 0.914536 |
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Target: 5'- aGGGUUcCCACGagUCGGGCguuucgUCGGGu -3' miRNA: 3'- -UCCAA-GGUGCgaAGCUCGaca---AGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 17405 | 0.66 | 0.901479 |
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Target: 5'- ----gCCGUGU-UCGGGCUGUUCGAGg -3' miRNA: 3'- uccaaGGUGCGaAGCUCGACAAGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 28826 | 0.66 | 0.901479 |
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Target: 5'- cGaGUUCUACGggU--GGCUGUUCGAGa -3' miRNA: 3'- uC-CAAGGUGCgaAgcUCGACAAGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 47325 | 0.67 | 0.879906 |
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Target: 5'- cGGcGUUCaucaGCgUCGGGCUGUUCGGc -3' miRNA: 3'- -UC-CAAGgug-CGaAGCUCGACAAGCUc -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 33763 | 0.67 | 0.859369 |
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Target: 5'- gAGGccUCC-CGCUgguucaUCGAGCUGUcaaacucgauauaguUCGAGu -3' miRNA: 3'- -UCCa-AGGuGCGA------AGCUCGACA---------------AGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 58129 | 0.67 | 0.847645 |
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Target: 5'- cGGGcUUCAUccgGCUgUCGAGCUGUUCGu- -3' miRNA: 3'- -UCCaAGGUG---CGA-AGCUCGACAAGCuc -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 24239 | 0.68 | 0.842485 |
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Target: 5'- aAGGUcacucaucgagcguuUCCACGCaUCGAGg-GUUCGGu -3' miRNA: 3'- -UCCA---------------AGGUGCGaAGCUCgaCAAGCUc -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 64034 | 0.73 | 0.547764 |
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Target: 5'- ---cUCUGCGCUUCGAccucGUUGUUCGAGa -3' miRNA: 3'- uccaAGGUGCGAAGCU----CGACAAGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 29965 | 0.74 | 0.505627 |
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Target: 5'- ---aUCUACGC--CGAGCUGUUCGGGg -3' miRNA: 3'- uccaAGGUGCGaaGCUCGACAAGCUC- -5' |
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| 12518 | 5' | -52.7 | NC_003345.1 | + | 12733 | 1.1 | 0.002317 |
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Target: 5'- gAGGUUCCACGCUUCGAGCUGUUCGAGg -3' miRNA: 3'- -UCCAAGGUGCGAAGCUCGACAAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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