Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1252 | 5' | -56.8 | NC_001317.1 | + | 28134 | 0.81 | 0.037018 |
Target: 5'- cGCCGUUUCAGC--AUGGGCGGCGcgcuGGCa -3' miRNA: 3'- -UGGCAAGGUCGagUACCCGCCGU----UCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 6076 | 0.66 | 0.453156 |
Target: 5'- uGCCGUUCUAuGCgcCGUGGGUcGCcGGUg -3' miRNA: 3'- -UGGCAAGGU-CGa-GUACCCGcCGuUCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 3580 | 0.66 | 0.463502 |
Target: 5'- aGCCGUcgcgcuguuUCCAGCUC-UGcaCGGuCGGGCg -3' miRNA: 3'- -UGGCA---------AGGUCGAGuACccGCC-GUUCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 410 | 1.1 | 0.000242 |
Target: 5'- uACCGUUCCAGCUCAUGGGCGGCAAGCc -3' miRNA: 3'- -UGGCAAGGUCGAGUACCCGCCGUUCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 1274 | 0.7 | 0.255332 |
Target: 5'- cGCUGUUgCAGCggGUGaGGUGGCA-GCa -3' miRNA: 3'- -UGGCAAgGUCGagUAC-CCGCCGUuCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 30577 | 0.68 | 0.3231 |
Target: 5'- aGCCGcgCCAGCUC-UGGcgacGCacaaccagaacgGGCAAGCc -3' miRNA: 3'- -UGGCaaGGUCGAGuACC----CG------------CCGUUCG- -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 2795 | 0.66 | 0.410167 |
Target: 5'- cGCCGguggCCAGCUC-UGcgacgauggccaguGGCGGCAGa- -3' miRNA: 3'- -UGGCaa--GGUCGAGuAC--------------CCGCCGUUcg -5' |
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1252 | 5' | -56.8 | NC_001317.1 | + | 9585 | 0.66 | 0.463502 |
Target: 5'- gGCUGUUgCGGUU--UGGGCGGUcuuugAGGCc -3' miRNA: 3'- -UGGCAAgGUCGAguACCCGCCG-----UUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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