miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12520 3' -59.6 NC_003345.1 + 14566 1.08 0.000854
Target:  5'- uCGCCAGCCGACGUUCCCGGUGGGUAGu -3'
miRNA:   3'- -GCGGUCGGCUGCAAGGGCCACCCAUC- -5'
12520 3' -59.6 NC_003345.1 + 29483 0.67 0.56245
Target:  5'- aG-CAGUCGGCGUccacuuugUCgucgauuucguCCGGUGGGUAGa -3'
miRNA:   3'- gCgGUCGGCUGCA--------AG-----------GGCCACCCAUC- -5'
12520 3' -59.6 NC_003345.1 + 38628 0.69 0.445359
Target:  5'- gCGCgGGCCGACucgGUUCCC-GUGGaUGGg -3'
miRNA:   3'- -GCGgUCGGCUG---CAAGGGcCACCcAUC- -5'
12520 3' -59.6 NC_003345.1 + 58226 0.76 0.165153
Target:  5'- uCGaCAGCCgGAUGaagCCCGGUGGGUGGu -3'
miRNA:   3'- -GCgGUCGG-CUGCaa-GGGCCACCCAUC- -5'
12520 3' -59.6 NC_003345.1 + 75716 0.67 0.56245
Target:  5'- uCGCCgcaguugcGGCgGAggcUGUUCUCGGUGGGggAGa -3'
miRNA:   3'- -GCGG--------UCGgCU---GCAAGGGCCACCCa-UC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.