miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12522 3' -53.5 NC_003345.1 + 15100 1.11 0.001881
Target:  5'- uCUCUGCUAUCUCGGUGGACUCGUCGCc -3'
miRNA:   3'- -GAGACGAUAGAGCCACCUGAGCAGCG- -5'
12522 3' -53.5 NC_003345.1 + 61332 0.7 0.680247
Target:  5'- gCUCcGCaAUCUCGaUGGACUUGcCGCc -3'
miRNA:   3'- -GAGaCGaUAGAGCcACCUGAGCaGCG- -5'
12522 3' -53.5 NC_003345.1 + 59479 0.7 0.701573
Target:  5'- gCUCUGCUGg-UCGGUGGugGCUaccUCGCu -3'
miRNA:   3'- -GAGACGAUagAGCCACC--UGAgc-AGCG- -5'
12522 3' -53.5 NC_003345.1 + 4553 0.67 0.838727
Target:  5'- ---cGCcgGUCgUCGGuUGGugUCGUUGCu -3'
miRNA:   3'- gagaCGa-UAG-AGCC-ACCugAGCAGCG- -5'
12522 3' -53.5 NC_003345.1 + 9889 0.66 0.871554
Target:  5'- gUC-GCUGUCgcugUCGGUgGGGCUgGUUGUg -3'
miRNA:   3'- gAGaCGAUAG----AGCCA-CCUGAgCAGCG- -5'
12522 3' -53.5 NC_003345.1 + 6952 0.66 0.878428
Target:  5'- uCUCUGCgaaggcugucUCUCGGUGuGGCUCaagucuucggcugGUCGg -3'
miRNA:   3'- -GAGACGau--------AGAGCCAC-CUGAG-------------CAGCg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.