Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12522 | 5' | -62.5 | NC_003345.1 | + | 24570 | 0.66 | 0.438439 |
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Target: 5'- gGAGCGGucGUCCuCgGCGGCaggacGCCGGGa -3' miRNA: 3'- -CUCGCUu-CGGGcGgCGCUGa----CGGCCC- -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 10845 | 0.66 | 0.429498 |
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Target: 5'- aGGGCGAGuCCUGCCGaacaAUcGCCGGGu -3' miRNA: 3'- -CUCGCUUcGGGCGGCgc--UGaCGGCCC- -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 62935 | 0.68 | 0.346365 |
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Target: 5'- gGAGCuGGAGUaguUCGCCGgGuCgagGCCGGGa -3' miRNA: 3'- -CUCG-CUUCG---GGCGGCgCuGa--CGGCCC- -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 30633 | 0.68 | 0.323778 |
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Target: 5'- aGGCGucccguGGCCCGugugggaguaCCGCaACUGCCGGa -3' miRNA: 3'- cUCGCu-----UCGGGC----------GGCGcUGACGGCCc -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 23174 | 0.69 | 0.316496 |
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Target: 5'- cGGCaGAAGUCCaacaGUCGCGGgaagucCUGCCGGGc -3' miRNA: 3'- cUCG-CUUCGGG----CGGCGCU------GACGGCCC- -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 23056 | 0.7 | 0.275408 |
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Target: 5'- ---gGAGGCCCGgcaggacuucCCGCGACUGuuGGa -3' miRNA: 3'- cucgCUUCGGGC----------GGCGCUGACggCCc -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 10215 | 0.7 | 0.268989 |
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Target: 5'- aAGuCGAAGcCCCGCCGCGACgUGUCu-- -3' miRNA: 3'- cUC-GCUUC-GGGCGGCGCUG-ACGGccc -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 44945 | 0.71 | 0.238698 |
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Target: 5'- cGAGCGAAGUacguucacgCCGCCGuUGACgGUCGGa -3' miRNA: 3'- -CUCGCUUCG---------GGCGGC-GCUGaCGGCCc -5' |
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| 12522 | 5' | -62.5 | NC_003345.1 | + | 15065 | 1.08 | 0.000401 |
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Target: 5'- gGAGCGAAGCCCGCCGCGACUGCCGGGc -3' miRNA: 3'- -CUCGCUUCGGGCGGCGCUGACGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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