miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12525 3' -53.1 NC_003345.1 + 16856 1.08 0.002934
Target:  5'- gCUAUCCUCGAUUCACUCUCCGGCCAGc -3'
miRNA:   3'- -GAUAGGAGCUAAGUGAGAGGCCGGUC- -5'
12525 3' -53.1 NC_003345.1 + 66394 0.79 0.245827
Target:  5'- --uUCCUCGAUUCcCUCaaUCCGGCCAc -3'
miRNA:   3'- gauAGGAGCUAAGuGAG--AGGCCGGUc -5'
12525 3' -53.1 NC_003345.1 + 4210 0.71 0.624597
Target:  5'- -cAUCCUCGAUUCGgUCUuCCGGggcgUCGGg -3'
miRNA:   3'- gaUAGGAGCUAAGUgAGA-GGCC----GGUC- -5'
12525 3' -53.1 NC_003345.1 + 3885 0.71 0.656202
Target:  5'- -cAUCCUcccaauaCGAUUC-CUCUCCGGCUu- -3'
miRNA:   3'- gaUAGGA-------GCUAAGuGAGAGGCCGGuc -5'
12525 3' -53.1 NC_003345.1 + 52632 0.67 0.864064
Target:  5'- -aGUCCUCcuuuugUCccgccCUCUCCGGUCGGg -3'
miRNA:   3'- gaUAGGAGcua---AGu----GAGAGGCCGGUC- -5'
12525 3' -53.1 NC_003345.1 + 6963 0.66 0.879662
Target:  5'- gCUGUCuCUCGGUguggcUCAagUCUUCGGCUGGu -3'
miRNA:   3'- -GAUAG-GAGCUA-----AGUg-AGAGGCCGGUC- -5'
12525 3' -53.1 NC_003345.1 + 60300 0.66 0.89426
Target:  5'- gCUA-CCUCGAUgcUCGCUa--UGGCCGGu -3'
miRNA:   3'- -GAUaGGAGCUA--AGUGAgagGCCGGUC- -5'
12525 3' -53.1 NC_003345.1 + 63525 0.66 0.89426
Target:  5'- -cAUCCUCGAU--GCUCUCuCGGagGGa -3'
miRNA:   3'- gaUAGGAGCUAagUGAGAG-GCCggUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.