Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12527 | 5' | -51.5 | NC_003345.1 | + | 44837 | 0.66 | 0.930846 |
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Target: 5'- uGCGGauauucagGAGAAGUccGAugGCCGg-- -3' miRNA: 3'- uCGCCa-------CUCUUCGuaCUugCGGUauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 50784 | 0.67 | 0.884148 |
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Target: 5'- cGGCGGUG-GAaacGGCggGGACGCUg--- -3' miRNA: 3'- -UCGCCACuCU---UCGuaCUUGCGGuauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 15108 | 0.68 | 0.851426 |
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Target: 5'- cGGCGGUcgaaagaaucGAGggGgAUGAguACGCCGc-- -3' miRNA: 3'- -UCGCCA----------CUCuuCgUACU--UGCGGUauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 24627 | 0.69 | 0.804204 |
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Target: 5'- cAGCGGUGGcGAauaucauAGC--GAGCGCCGUGGu -3' miRNA: 3'- -UCGCCACU-CU-------UCGuaCUUGCGGUAUU- -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 18681 | 0.69 | 0.795318 |
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Target: 5'- cGGuCGGUGGGAuGGCAUGGGaGCCGg-- -3' miRNA: 3'- -UC-GCCACUCU-UCGUACUUgCGGUauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 55705 | 0.69 | 0.795318 |
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Target: 5'- cGGCGGUGGuGggGCggGUGAacccggugACGCCGa-- -3' miRNA: 3'- -UCGCCACU-CuuCG--UACU--------UGCGGUauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 44989 | 0.7 | 0.721937 |
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Target: 5'- aAGCGGUGAGGAGCA--GACGauuCCGa-- -3' miRNA: 3'- -UCGCCACUCUUCGUacUUGC---GGUauu -5' |
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| 12527 | 5' | -51.5 | NC_003345.1 | + | 18754 | 1.06 | 0.004395 |
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Target: 5'- cAGCGGUGAGAAGCAUGAACGCCAUAAc -3' miRNA: 3'- -UCGCCACUCUUCGUACUUGCGGUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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