Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1253 | 5' | -52.3 | NC_001317.1 | + | 18213 | 0.66 | 0.72407 |
Target: 5'- gUCGCAGUGggGgCGGCUauuguGGCUGGUg- -3' miRNA: 3'- -GGUGUCGCaaUgGCCGG-----UCGAUUAgu -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 817 | 0.66 | 0.72407 |
Target: 5'- cCCGCGGCGgguguauuugGCCggugaGGCCAGUagcuUGAUUAg -3' miRNA: 3'- -GGUGUCGCaa--------UGG-----CCGGUCG----AUUAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 9926 | 0.66 | 0.689611 |
Target: 5'- cCCACcauguGCGcgaacgACCGGCgCGGgUGGUCAg -3' miRNA: 3'- -GGUGu----CGCaa----UGGCCG-GUCgAUUAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 13368 | 0.67 | 0.6663 |
Target: 5'- gCACAGCGcgGCgcacaGGCCAGCa----- -3' miRNA: 3'- gGUGUCGCaaUGg----CCGGUCGauuagu -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 15938 | 0.67 | 0.654583 |
Target: 5'- gCCAgCAGUGcugACCGGUCAGggAAUUAc -3' miRNA: 3'- -GGU-GUCGCaa-UGGCCGGUCgaUUAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 17033 | 0.68 | 0.588877 |
Target: 5'- cCCGCAGCGccugcugcggccaugUUACCGGCaAGCUc---- -3' miRNA: 3'- -GGUGUCGC---------------AAUGGCCGgUCGAuuagu -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 6051 | 0.69 | 0.515431 |
Target: 5'- -uGCGGCGgucAUCGGCCAGCUuaacCAg -3' miRNA: 3'- ggUGUCGCaa-UGGCCGGUCGAuua-GU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 19072 | 0.71 | 0.41926 |
Target: 5'- gUACAGUG-UACCGGCaAGCUGuAUCGa -3' miRNA: 3'- gGUGUCGCaAUGGCCGgUCGAU-UAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 8367 | 0.74 | 0.285763 |
Target: 5'- gCuCAGUGUUGCCGGUCAGCUc---- -3' miRNA: 3'- gGuGUCGCAAUGGCCGGUCGAuuagu -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 18253 | 0.75 | 0.24938 |
Target: 5'- cCCACuGCGacaAUCGGCCAGgUAAUCGc -3' miRNA: 3'- -GGUGuCGCaa-UGGCCGGUCgAUUAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 14858 | 0.75 | 0.242579 |
Target: 5'- gCAgGGCGUUACCGGCauCAGCgccUCAg -3' miRNA: 3'- gGUgUCGCAAUGGCCG--GUCGauuAGU- -5' |
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1253 | 5' | -52.3 | NC_001317.1 | + | 910 | 1.11 | 0.000642 |
Target: 5'- gCCACAGCGUUACCGGCCAGCUAAUCAa -3' miRNA: 3'- -GGUGUCGCAAUGGCCGGUCGAUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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