Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12530 | 3' | -55.5 | NC_003345.1 | + | 6211 | 0.66 | 0.75473 |
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Target: 5'- cAUCGAG-GGGAuGGCgaAGuauGCCUGCUCu -3' miRNA: 3'- -UAGCUCgCCCUcUUG--UCu--CGGAUGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 31643 | 0.66 | 0.75473 |
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Target: 5'- cAUCGAGgGGGAgGAACGaaucGAGCgUaaucGCUCu -3' miRNA: 3'- -UAGCUCgCCCU-CUUGU----CUCGgA----UGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 60102 | 0.66 | 0.748582 |
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Target: 5'- uUC-AGuUGGGAGAGCuGAGCCUgagcguuccgccacgACUCg -3' miRNA: 3'- uAGcUC-GCCCUCUUGuCUCGGA---------------UGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 23554 | 0.67 | 0.70238 |
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Target: 5'- cAUCGAGCGGuauugcGAGGGCGacGGCCUGUUCg -3' miRNA: 3'- -UAGCUCGCC------CUCUUGUc-UCGGAUGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 45978 | 0.67 | 0.70238 |
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Target: 5'- -aCGGGCGGGuguGAGCcu-GCCUACa- -3' miRNA: 3'- uaGCUCGCCCu--CUUGucuCGGAUGag -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 38266 | 0.69 | 0.550919 |
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Target: 5'- aAUCGAGCGuaucgggucGGAGAuu-GAGuCCUACUCc -3' miRNA: 3'- -UAGCUCGC---------CCUCUuguCUC-GGAUGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 31060 | 0.74 | 0.317346 |
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Target: 5'- -cCGAGaGGGAGGACGG-GCCUuccGCUCg -3' miRNA: 3'- uaGCUCgCCCUCUUGUCuCGGA---UGAG- -5' |
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| 12530 | 3' | -55.5 | NC_003345.1 | + | 22319 | 1.08 | 0.001372 |
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Target: 5'- cAUCGAGCGGGAGAACAGAGCCUACUCg -3' miRNA: 3'- -UAGCUCGCCCUCUUGUCUCGGAUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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