Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12532 | 3' | -53.7 | NC_003345.1 | + | 32444 | 0.66 | 0.87773 |
Target: 5'- cCGUACuCGGgGAAUCcuucgccccggUCGUC-CGuCUCAa -3' miRNA: 3'- -GCAUG-GCCgCUUAG-----------AGCAGuGC-GAGU- -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 46825 | 0.67 | 0.862205 |
Target: 5'- uGUA-CGGCGAGcga-GUCACGCUCc -3' miRNA: 3'- gCAUgGCCGCUUagagCAGUGCGAGu -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 34099 | 0.67 | 0.854094 |
Target: 5'- ----gCGGCGAAUgUCGUCGCuuUCAc -3' miRNA: 3'- gcaugGCCGCUUAgAGCAGUGcgAGU- -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 59723 | 0.67 | 0.828458 |
Target: 5'- uGUACCGggcgcucgcGCGGuuaUCGUCAUGUUCAc -3' miRNA: 3'- gCAUGGC---------CGCUuagAGCAGUGCGAGU- -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 75634 | 0.69 | 0.73164 |
Target: 5'- ----gCGGCGAGcuguUC-CGUCGCGCUCGc -3' miRNA: 3'- gcaugGCCGCUU----AGaGCAGUGCGAGU- -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 15710 | 0.74 | 0.468804 |
Target: 5'- aGUucugCGGUGAAUCUC-UCACGCUCGa -3' miRNA: 3'- gCAug--GCCGCUUAGAGcAGUGCGAGU- -5' |
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12532 | 3' | -53.7 | NC_003345.1 | + | 23021 | 1.09 | 0.002354 |
Target: 5'- aCGUACCGGCGAAUCUCGUCACGCUCAu -3' miRNA: 3'- -GCAUGGCCGCUUAGAGCAGUGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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