Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12535 | 3' | -55 | NC_003345.1 | + | 21534 | 0.65 | 0.852457 |
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Target: 5'- -aCCACGUCgUGACCggCGGgaguGAUgACCu -3' miRNA: 3'- agGGUGCGG-ACUGGa-GCCau--CUA-UGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 27121 | 0.66 | 0.808888 |
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Target: 5'- gUCgCCGCG-CUGAagUCUCaGGUAGAcGCCg -3' miRNA: 3'- -AG-GGUGCgGACU--GGAG-CCAUCUaUGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 62945 | 0.66 | 0.808888 |
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Target: 5'- gCCUACG-CUGGCCUCcGGgcGAaGCUc -3' miRNA: 3'- aGGGUGCgGACUGGAG-CCauCUaUGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 32462 | 0.67 | 0.799614 |
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Target: 5'- uUCCCcgACGCC-GACCUCGaUGcGGUgaGCCa -3' miRNA: 3'- -AGGG--UGCGGaCUGGAGCcAU-CUA--UGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 6788 | 0.69 | 0.678362 |
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Target: 5'- cCCCACGCuuCUGAcgacaaccCCUucCGGUcGAUGCCu -3' miRNA: 3'- aGGGUGCG--GACU--------GGA--GCCAuCUAUGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 45321 | 0.69 | 0.667743 |
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Target: 5'- aCCCACGUCgu-CCUCGcUAacGAUACCg -3' miRNA: 3'- aGGGUGCGGacuGGAGCcAU--CUAUGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 19850 | 0.7 | 0.614366 |
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Target: 5'- -aCCGCGaCUaccacGACCUCGGUGGG-GCCg -3' miRNA: 3'- agGGUGCgGA-----CUGGAGCCAUCUaUGG- -5' |
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| 12535 | 3' | -55 | NC_003345.1 | + | 25752 | 1.13 | 0.000946 |
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Target: 5'- uUCCCACGCCUGACCUCGGUAGAUACCg -3' miRNA: 3'- -AGGGUGCGGACUGGAGCCAUCUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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