Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12537 | 3' | -47.2 | NC_003345.1 | + | 77317 | 0.68 | 0.991097 |
Target: 5'- uCCAGucGggGuCGGAUGggGAGGggUCGGc -3' miRNA: 3'- -GGUUuuCaaC-GCCUACa-CUCCa-AGCU- -5' |
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12537 | 3' | -47.2 | NC_003345.1 | + | 43218 | 0.7 | 0.965515 |
Target: 5'- cCCcGAAGUUGUGGAUGcUGGcGUUCa- -3' miRNA: 3'- -GGuUUUCAACGCCUAC-ACUcCAAGcu -5' |
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12537 | 3' | -47.2 | NC_003345.1 | + | 8833 | 0.7 | 0.95785 |
Target: 5'- cCCGAGAGUaccgaGUGGucUGUGAGGUguauagucUCGAa -3' miRNA: 3'- -GGUUUUCAa----CGCCu-ACACUCCA--------AGCU- -5' |
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12537 | 3' | -47.2 | NC_003345.1 | + | 28583 | 1.13 | 0.006558 |
Target: 5'- gCCAAAAGUUGCGGAUGUGAGGUUCGAg -3' miRNA: 3'- -GGUUUUCAACGCCUACACUCCAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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