Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12538 | 3' | -53.3 | NC_003345.1 | + | 19117 | 0.66 | 0.886177 |
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Target: 5'- -uUGGCGAgcgucgUCCUaaUCAUCCGCUUCu- -3' miRNA: 3'- uuACUGCUg-----AGGA--AGUAGGCGGAGcu -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 32651 | 0.66 | 0.876382 |
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Target: 5'- -uUGGCGAacucggugagcaucUUCCgcUCGUCCGgCUCGAa -3' miRNA: 3'- uuACUGCU--------------GAGGa-AGUAGGCgGAGCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 46927 | 0.66 | 0.854704 |
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Target: 5'- -----gGACUCCUgagcgUCAaCUGCCUCGAu -3' miRNA: 3'- uuacugCUGAGGA-----AGUaGGCGGAGCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 26486 | 0.66 | 0.854704 |
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Target: 5'- -uUGugGAUUCCcUC-UCCGCUuUCGAu -3' miRNA: 3'- uuACugCUGAGGaAGuAGGCGG-AGCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 50336 | 0.68 | 0.800759 |
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Target: 5'- -cUGGCGGCUCCUUCGggggUCGCggUGGu -3' miRNA: 3'- uuACUGCUGAGGAAGUa---GGCGgaGCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 52012 | 0.7 | 0.654606 |
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Target: 5'- --cGACGAgUCUaUCAUCCGgCUUGAc -3' miRNA: 3'- uuaCUGCUgAGGaAGUAGGCgGAGCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 35914 | 0.71 | 0.621743 |
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Target: 5'- --gGGCGACUCCUUC--CCGCuCUaCGAg -3' miRNA: 3'- uuaCUGCUGAGGAAGuaGGCG-GA-GCU- -5' |
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| 12538 | 3' | -53.3 | NC_003345.1 | + | 28837 | 1.07 | 0.003183 |
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Target: 5'- gAAUGACGACUCCUUCAUCCGCCUCGAu -3' miRNA: 3'- -UUACUGCUGAGGAAGUAGGCGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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