miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1254 3' -53.2 NC_001317.1 + 8601 0.66 0.624889
Target:  5'- gGCGCaggCUGUucgggUGUGCAgccgacgCACAGCaUCAg -3'
miRNA:   3'- aCGCGa--GACG-----ACAUGUa------GUGUCG-AGU- -5'
1254 3' -53.2 NC_001317.1 + 14366 0.68 0.485965
Target:  5'- gGCGCUCUGCacauugGUA-AUCAguacCGGCUCGu -3'
miRNA:   3'- aCGCGAGACGa-----CAUgUAGU----GUCGAGU- -5'
1254 3' -53.2 NC_001317.1 + 9355 0.7 0.354209
Target:  5'- aUGCGCUgCUGCUG-ACugguaCGCAGCUUu -3'
miRNA:   3'- -ACGCGA-GACGACaUGua---GUGUCGAGu -5'
1254 3' -53.2 NC_001317.1 + 1061 1.09 0.000621
Target:  5'- cUGCGCUCUGCUGUACAUCACAGCUCAc -3'
miRNA:   3'- -ACGCGAGACGACAUGUAGUGUCGAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.