Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12543 | 5' | -55.9 | NC_003345.1 | + | 41371 | 0.66 | 0.780418 |
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Target: 5'- uAGcauCCCGACUCGAUGcGGAUaUGAGu -3' miRNA: 3'- -UCacuGGGCUGAGCUAC-CCUGaGCUU- -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 19848 | 0.66 | 0.780418 |
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Target: 5'- --cGACCgCGACuaccacgaccUCGGUGGGGC-CGAu -3' miRNA: 3'- ucaCUGG-GCUG----------AGCUACCCUGaGCUu -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 62555 | 0.66 | 0.780418 |
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Target: 5'- cGGUGuCCCGAgUCGAUGcGACgacugCGGc -3' miRNA: 3'- -UCACuGGGCUgAGCUACcCUGa----GCUu -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 71450 | 0.66 | 0.770633 |
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Target: 5'- --cGcCCCGACUCuc-GGGACUUGAc -3' miRNA: 3'- ucaCuGGGCUGAGcuaCCCUGAGCUu -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 59321 | 0.66 | 0.750668 |
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Target: 5'- --cGGCCCGAC-CGAgcagaagaugUGGGACguagCGAc -3' miRNA: 3'- ucaCUGGGCUGaGCU----------ACCCUGa---GCUu -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 42018 | 0.67 | 0.719886 |
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Target: 5'- ---cGCCCGACUCG-UGGGAacccuucaUCGGAg -3' miRNA: 3'- ucacUGGGCUGAGCuACCCUg-------AGCUU- -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 22873 | 0.67 | 0.698933 |
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Target: 5'- --aGGCCCGGCagGAagcGGGACUCGu- -3' miRNA: 3'- ucaCUGGGCUGagCUa--CCCUGAGCuu -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 12751 | 0.67 | 0.676669 |
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Target: 5'- cGGUGuucCCaCGACUCGAUGcuaucaaacagauGGugUCGAAc -3' miRNA: 3'- -UCACu--GG-GCUGAGCUAC-------------CCugAGCUU- -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 22981 | 0.69 | 0.560225 |
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Target: 5'- --cGGCCCGAa-CGGUGGGACgUUGAAu -3' miRNA: 3'- ucaCUGGGCUgaGCUACCCUG-AGCUU- -5' |
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| 12543 | 5' | -55.9 | NC_003345.1 | + | 32347 | 1.06 | 0.002197 |
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Target: 5'- gAGUGACCCGACUCGAUGGGACUCGAAc -3' miRNA: 3'- -UCACUGGGCUGAGCUACCCUGAGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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