Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12545 | 3' | -55.8 | NC_003345.1 | + | 73826 | 0.66 | 0.8043 |
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Target: 5'- aUCUCGUCGGCGU---UCUUGAacgucGGCGu -3' miRNA: 3'- -AGAGCGGUCGCAcaaGGAACU-----CCGCc -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 68471 | 0.67 | 0.715366 |
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Target: 5'- aUCUCGCCcggcucggucGCG-GggCCacccgUGGGGCGGg -3' miRNA: 3'- -AGAGCGGu---------CGCaCaaGGa----ACUCCGCC- -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 33336 | 0.67 | 0.70496 |
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Target: 5'- aCUCGauuacgaCGGCGUGUUCCgcGAGcuuGUGGa -3' miRNA: 3'- aGAGCg------GUCGCACAAGGaaCUC---CGCC- -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 63330 | 0.7 | 0.577674 |
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Target: 5'- cCUCgGCCAGCGUGc-CCguguuaGAGGCGa -3' miRNA: 3'- aGAG-CGGUCGCACaaGGaa----CUCCGCc -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 55712 | 0.71 | 0.485219 |
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Target: 5'- aCUCGuCCGGCG-GUgg--UGGGGCGGg -3' miRNA: 3'- aGAGC-GGUCGCaCAaggaACUCCGCC- -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 70175 | 0.73 | 0.374312 |
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Target: 5'- aUCUCGCggaAGCGUGagcUUCUUgcugaguucgcUGAGGCGGa -3' miRNA: 3'- -AGAGCGg--UCGCAC---AAGGA-----------ACUCCGCC- -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 65033 | 0.74 | 0.365872 |
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Target: 5'- cCUCGCCAGCGUa-UCCUcggggaaGGGGUGGu -3' miRNA: 3'- aGAGCGGUCGCAcaAGGAa------CUCCGCC- -5' |
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| 12545 | 3' | -55.8 | NC_003345.1 | + | 33484 | 1.12 | 0.000975 |
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Target: 5'- aUCUCGCCAGCGUGUUCCUUGAGGCGGa -3' miRNA: 3'- -AGAGCGGUCGCACAAGGAACUCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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