Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1255 | 5' | -52.3 | NC_001317.1 | + | 10351 | 0.66 | 0.771705 |
Target: 5'- aCC-ACCGUgACCGUggUCGUCGAgcugcacACCGu -3' miRNA: 3'- -GGcUGGCAgUGGCG--AGUAGUUa------UGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 17795 | 0.66 | 0.771705 |
Target: 5'- uUGACCGgcUCACCGCUgCGUUuaaaGCCu -3' miRNA: 3'- gGCUGGC--AGUGGCGA-GUAGuua-UGGc -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 19978 | 0.66 | 0.771705 |
Target: 5'- aCGAUgGUgACCG-UCAUCAGUuugcuauuGCCGa -3' miRNA: 3'- gGCUGgCAgUGGCgAGUAGUUA--------UGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 19973 | 0.66 | 0.761062 |
Target: 5'- gCUGGCCGUCAUCGCac-----UACCGg -3' miRNA: 3'- -GGCUGGCAGUGGCGaguaguuAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 17064 | 0.66 | 0.739355 |
Target: 5'- cCCGGCCaUCGuuCCGUgUcgCGAUACCGa -3' miRNA: 3'- -GGCUGGcAGU--GGCGaGuaGUUAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 15839 | 0.67 | 0.717174 |
Target: 5'- gUCGACCGUCuggaacagcaccGCCGCgcaAUCGGgcUGCUGu -3' miRNA: 3'- -GGCUGGCAG------------UGGCGag-UAGUU--AUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 9192 | 0.67 | 0.67181 |
Target: 5'- aCCGGgCGcuUCACCaGCUCAggCAGUGgCGg -3' miRNA: 3'- -GGCUgGC--AGUGG-CGAGUa-GUUAUgGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 14388 | 0.68 | 0.660334 |
Target: 5'- gCCGACCagucaaagccuGUCACCGUugUCGUgCGcguUGCCGa -3' miRNA: 3'- -GGCUGG-----------CAGUGGCG--AGUA-GUu--AUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 4553 | 0.68 | 0.660334 |
Target: 5'- aCGGCgGUCACCGCUuCAU--GUACa- -3' miRNA: 3'- gGCUGgCAGUGGCGA-GUAguUAUGgc -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 2833 | 0.68 | 0.625784 |
Target: 5'- gCCGACCG-CAUCGaaauugaCAUCAGUcACCGc -3' miRNA: 3'- -GGCUGGCaGUGGCga-----GUAGUUA-UGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 10114 | 0.68 | 0.63731 |
Target: 5'- uCUGACCGUCAgugcacuggucuCCgGCUCGuacUCAAUgACCGc -3' miRNA: 3'- -GGCUGGCAGU------------GG-CGAGU---AGUUA-UGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 23773 | 0.69 | 0.579849 |
Target: 5'- --uACCG-CACCGCUCGcCAGUucACCGa -3' miRNA: 3'- ggcUGGCaGUGGCGAGUaGUUA--UGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 14381 | 0.69 | 0.591286 |
Target: 5'- aCCGGCUuUCGCaauggCGCUCugcacauugguaAUCAGUACCGg -3' miRNA: 3'- -GGCUGGcAGUG-----GCGAG------------UAGUUAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 6144 | 0.71 | 0.480074 |
Target: 5'- aUGACCGUCauGCUGUggCGUCucGAUGCCGg -3' miRNA: 3'- gGCUGGCAG--UGGCGa-GUAG--UUAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 13190 | 0.72 | 0.389424 |
Target: 5'- aCCGccGCCGUCAUCGC-CAUUAcugaugcgaAUACCGu -3' miRNA: 3'- -GGC--UGGCAGUGGCGaGUAGU---------UAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 2290 | 0.73 | 0.361793 |
Target: 5'- -aGGCCGagGCCGguugCGUCAAUGCCGa -3' miRNA: 3'- ggCUGGCagUGGCga--GUAGUUAUGGC- -5' |
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1255 | 5' | -52.3 | NC_001317.1 | + | 1455 | 1.13 | 0.000664 |
Target: 5'- aCCGACCGUCACCGCUCAUCAAUACCGg -3' miRNA: 3'- -GGCUGGCAGUGGCGAGUAGUUAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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