Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12554 | 5' | -56.9 | NC_003345.1 | + | 37721 | 0.66 | 0.714482 |
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Target: 5'- cGAUacACgCCGGGCCGAGUccCCCGuGa -3' miRNA: 3'- aCUAa-UG-GGCUCGGCUCGcaGGGCuC- -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 75242 | 0.67 | 0.693754 |
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Target: 5'- gGAaaGCuCCGAGUauAGCGUCuCCGAGg -3' miRNA: 3'- aCUaaUG-GGCUCGgcUCGCAG-GGCUC- -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 60107 | 0.67 | 0.683303 |
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Target: 5'- uUGGgagAgCUGAGCCuGAGCGUUCCGc- -3' miRNA: 3'- -ACUaa-UgGGCUCGG-CUCGCAGGGCuc -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 35821 | 0.67 | 0.677009 |
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Target: 5'- cGAUgaaGCCCGcauuguggaugaacaGGCCGAGCcG-CCCGAc -3' miRNA: 3'- aCUAa--UGGGC---------------UCGGCUCG-CaGGGCUc -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 42114 | 0.67 | 0.662273 |
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Target: 5'- gGGUUcCCaCGAGUCGGGCGUUUCGu- -3' miRNA: 3'- aCUAAuGG-GCUCGGCUCGCAGGGCuc -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 2780 | 0.68 | 0.630558 |
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Target: 5'- cGAgcuuCCCGAGCCGgAGguUCCCGAu -3' miRNA: 3'- aCUaau-GGGCUCGGC-UCgcAGGGCUc -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 66090 | 0.69 | 0.536346 |
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Target: 5'- gGAcauUCCGAGCgguuggGAGCGUCCCGAc -3' miRNA: 3'- aCUaauGGGCUCGg-----CUCGCAGGGCUc -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 62569 | 0.7 | 0.476262 |
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Target: 5'- gGAcUACCgCGuGUCG-GUGUCCCGAGu -3' miRNA: 3'- aCUaAUGG-GCuCGGCuCGCAGGGCUC- -5' |
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| 12554 | 5' | -56.9 | NC_003345.1 | + | 38115 | 1.09 | 0.001153 |
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Target: 5'- gUGAUUACCCGAGCCGAGCGUCCCGAGa -3' miRNA: 3'- -ACUAAUGGGCUCGGCUCGCAGGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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