Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12556 | 3' | -53.2 | NC_003345.1 | + | 14579 | 0.66 | 0.897898 |
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Target: 5'- cGGcgCUCGUGGGUCGccaGCCgacguUCCcGGugGg -3' miRNA: 3'- -UCa-GAGCACCCAGU---UGG-----AGGuUCugU- -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 69752 | 0.66 | 0.897898 |
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Target: 5'- --gCUCGUacuGGUCGgcaaGCCUCCAcGACAa -3' miRNA: 3'- ucaGAGCAc--CCAGU----UGGAGGUuCUGU- -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 12374 | 0.66 | 0.868157 |
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Target: 5'- -cUCUCGUGgaGGUCAagaaguACCUUCGAGAg- -3' miRNA: 3'- ucAGAGCAC--CCAGU------UGGAGGUUCUgu -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 11137 | 0.68 | 0.807132 |
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Target: 5'- -aUCUCGUGGGaUUAcgaacGCUUCgAGGACAu -3' miRNA: 3'- ucAGAGCACCC-AGU-----UGGAGgUUCUGU- -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 75897 | 0.69 | 0.716252 |
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Target: 5'- aGGUCguaUCGUgccGGGUCuuCCUCCuuGGACGg -3' miRNA: 3'- -UCAG---AGCA---CCCAGuuGGAGGu-UCUGU- -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 20521 | 0.7 | 0.694868 |
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Target: 5'- -cUCUaCGUGGGUCAACCgCUucGAUAa -3' miRNA: 3'- ucAGA-GCACCCAGUUGGaGGuuCUGU- -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 52195 | 0.77 | 0.318027 |
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Target: 5'- gAGUCUCGUGGGUCAgcgaaaaGCCUUCGGaGCc -3' miRNA: 3'- -UCAGAGCACCCAGU-------UGGAGGUUcUGu -5' |
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| 12556 | 3' | -53.2 | NC_003345.1 | + | 39919 | 1.09 | 0.002386 |
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Target: 5'- aAGUCUCGUGGGUCAACCUCCAAGACAa -3' miRNA: 3'- -UCAGAGCACCCAGUUGGAGGUUCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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