Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12556 | 5' | -54.5 | NC_003345.1 | + | 18328 | 0.66 | 0.822596 |
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Target: 5'- --uGUCCCguuGAGGGAUGuGaCCACGAAg -3' miRNA: 3'- uugUAGGG---CUUCCUGCuCaGGUGCUU- -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 69781 | 0.67 | 0.774699 |
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Target: 5'- uGGCGUCCUGAuGGAgGAcGUgCGCGAc -3' miRNA: 3'- -UUGUAGGGCUuCCUgCU-CAgGUGCUu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 68654 | 0.67 | 0.744128 |
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Target: 5'- cGCAUCCCGGucgccaucGGGAUGugguAGUCCAUGu- -3' miRNA: 3'- uUGUAGGGCU--------UCCUGC----UCAGGUGCuu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 31065 | 0.68 | 0.712548 |
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Target: 5'- -uCGUCCCGAgagggAGGACGGGccuUCCGCu-- -3' miRNA: 3'- uuGUAGGGCU-----UCCUGCUC---AGGUGcuu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 29051 | 0.68 | 0.695398 |
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Target: 5'- uGCAUCCCG-AGGACGGccuugcggaugauguGUCgGCGGu -3' miRNA: 3'- uUGUAGGGCuUCCUGCU---------------CAGgUGCUu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 10834 | 0.68 | 0.669397 |
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Target: 5'- cGACGagaCGGAGGGCGAGUCCugcCGAAc -3' miRNA: 3'- -UUGUaggGCUUCCUGCUCAGGu--GCUU- -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 38100 | 0.69 | 0.658496 |
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Target: 5'- gAGCGUCCCGAGagagcGGauuGCGuAGUCCACGu- -3' miRNA: 3'- -UUGUAGGGCUU-----CC---UGC-UCAGGUGCuu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 55816 | 0.7 | 0.571237 |
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Target: 5'- --aGUCaCCGAAGGugGAGUCCuCGc- -3' miRNA: 3'- uugUAG-GGCUUCCugCUCAGGuGCuu -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 60550 | 0.73 | 0.437433 |
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Target: 5'- uAACGUCCCGuGGGGCGGuguGUCCuCGGAu -3' miRNA: 3'- -UUGUAGGGCuUCCUGCU---CAGGuGCUU- -5' |
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| 12556 | 5' | -54.5 | NC_003345.1 | + | 39885 | 1.04 | 0.003443 |
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Target: 5'- gAACAUCCCGAAGGACGAGUCCACGAAg -3' miRNA: 3'- -UUGUAGGGCUUCCUGCUCAGGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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