Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12557 | 5' | -55.8 | NC_003345.1 | + | 35999 | 0.66 | 0.796455 |
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Target: 5'- gGAGUCGCcccggUCuGGUCGCUGUAUUu -3' miRNA: 3'- gCUCGGCGca---AGcUCGGCGACAUAGu -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 34026 | 0.66 | 0.771425 |
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Target: 5'- uGAGCCGUGUUCGccuucuuacacggguAGCCGgaGa---- -3' miRNA: 3'- gCUCGGCGCAAGC---------------UCGGCgaCauagu -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 33744 | 0.66 | 0.766501 |
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Target: 5'- uCGAGCUGUcaaacucgauauaGUUCGAGUCGgUGUcgCc -3' miRNA: 3'- -GCUCGGCG-------------CAAGCUCGGCgACAuaGu -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 32893 | 0.66 | 0.757555 |
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Target: 5'- uGAGCgGCGUuaUCGAGugacCCGCUGaccuUCGu -3' miRNA: 3'- gCUCGgCGCA--AGCUC----GGCGACau--AGU- -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 52176 | 0.66 | 0.757555 |
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Target: 5'- uCGAGUCGgGUUCucGCCGgaGUcUCGu -3' miRNA: 3'- -GCUCGGCgCAAGcuCGGCgaCAuAGU- -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 69622 | 0.67 | 0.727072 |
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Target: 5'- uCGAGCCGaCGacccaUCGuGCCGUUGUcgUg -3' miRNA: 3'- -GCUCGGC-GCa----AGCuCGGCGACAuaGu -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 20108 | 0.71 | 0.485738 |
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Target: 5'- -cAGCCGgGUUCGGGCCGUucucggUGUacGUCGg -3' miRNA: 3'- gcUCGGCgCAAGCUCGGCG------ACA--UAGU- -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 21802 | 0.72 | 0.418697 |
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Target: 5'- gCGAGCCGC--UgGAGCCGCUGa---- -3' miRNA: 3'- -GCUCGGCGcaAgCUCGGCGACauagu -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 1136 | 0.76 | 0.268099 |
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Target: 5'- aGGGCCG-GUUCGGGCCGCUu--UCAg -3' miRNA: 3'- gCUCGGCgCAAGCUCGGCGAcauAGU- -5' |
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| 12557 | 5' | -55.8 | NC_003345.1 | + | 40158 | 1.09 | 0.001314 |
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Target: 5'- aCGAGCCGCGUUCGAGCCGCUGUAUCAa -3' miRNA: 3'- -GCUCGGCGCAAGCUCGGCGACAUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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