Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1256 | 3' | -52.5 | NC_001317.1 | + | 16 | 0.74 | 0.244202 |
Target: 5'- -gCAuugCGCGCCAGAggUGGCGCGu- -3' miRNA: 3'- ugGUuuaGCGCGGUCUa-ACCGCGUug -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 1806 | 0.7 | 0.413705 |
Target: 5'- aGCUgcGUUGCGCCAaucUGGCGCGAUu -3' miRNA: 3'- -UGGuuUAGCGCGGUcuaACCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 1867 | 1.1 | 0.000588 |
Target: 5'- cACCAAAUCGCGCCAGAUUGGCGCAACg -3' miRNA: 3'- -UGGUUUAGCGCGGUCUAACCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 3265 | 0.91 | 0.015212 |
Target: 5'- cUCAAAUCcCGCCAGAUUGGCGCGACg -3' miRNA: 3'- uGGUUUAGcGCGGUCUAACCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 4430 | 0.66 | 0.674291 |
Target: 5'- uCCAGuUCG-GCCAGcgUGGCGgCGGa -3' miRNA: 3'- uGGUUuAGCgCGGUCuaACCGC-GUUg -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 7272 | 0.71 | 0.365143 |
Target: 5'- gACaCGAuUUGCGCCAGGUauuacgGGCGCAc- -3' miRNA: 3'- -UG-GUUuAGCGCGGUCUAa-----CCGCGUug -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 8240 | 0.7 | 0.413704 |
Target: 5'- cGCCAGccgUGCGCCAGAU-GGUGacCAGCu -3' miRNA: 3'- -UGGUUua-GCGCGGUCUAaCCGC--GUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 9667 | 0.75 | 0.200172 |
Target: 5'- cACCGGAg-GCGCguGAuUUGGCGCGGCu -3' miRNA: 3'- -UGGUUUagCGCGguCU-AACCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 14828 | 0.66 | 0.65063 |
Target: 5'- gACCGAGU-GCGCgGGcggUGGCGUAu- -3' miRNA: 3'- -UGGUUUAgCGCGgUCua-ACCGCGUug -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 16899 | 0.68 | 0.510277 |
Target: 5'- cACUGGcgCGCGUCAGu---GCGCAGCa -3' miRNA: 3'- -UGGUUuaGCGCGGUCuaacCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 23449 | 0.67 | 0.603171 |
Target: 5'- -aCGAAgCGUGCCGGGcuUUuGCGCAGCg -3' miRNA: 3'- ugGUUUaGCGCGGUCU--AAcCGCGUUG- -5' |
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1256 | 3' | -52.5 | NC_001317.1 | + | 30511 | 0.68 | 0.505771 |
Target: 5'- cCCGuucugguugugCGuCGCCAGAgcUGGCGCGGCu -3' miRNA: 3'- uGGUuua--------GC-GCGGUCUa-ACCGCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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