Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1256 | 5' | -58.8 | NC_001317.1 | + | 9190 | 0.66 | 0.32045 |
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Target: 5'- gAGCCaGUGGGaugagugaauuaaccGCGCUGCagGA-GCGCc -3' miRNA: 3'- aUCGG-CACUC---------------CGCGACGagCUaCGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 16115 | 0.67 | 0.309247 |
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Target: 5'- cUGGCgGcgGuGGCGCaGUccgaggUCGAUGCGCu -3' miRNA: 3'- -AUCGgCa-CuCCGCGaCG------AGCUACGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 6088 | 0.67 | 0.293766 |
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Target: 5'- -cGCCGUGGGucgccgguGUGCUGaCUgauggucgUGGUGCGCa -3' miRNA: 3'- auCGGCACUC--------CGCGAC-GA--------GCUACGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 16981 | 0.67 | 0.286254 |
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Target: 5'- aUGGCCGcagcAGGCGCUGCggggUCGGUauCGCg -3' miRNA: 3'- -AUCGGCac--UCCGCGACG----AGCUAc-GCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 8669 | 0.68 | 0.26463 |
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Target: 5'- -cGCCGUcuugagGGGGCGCUGaCUgccUGCGCg -3' miRNA: 3'- auCGGCA------CUCCGCGAC-GAgcuACGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 18748 | 0.69 | 0.219335 |
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Target: 5'- -uGCCGgGAGGUGCUGCgcCGggGCa- -3' miRNA: 3'- auCGGCaCUCCGCGACGa-GCuaCGcg -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 862 | 0.7 | 0.190645 |
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Target: 5'- cUGGCCGguaacgcUGuGGCGCUGCUCaaGAaagGCGUu -3' miRNA: 3'- -AUCGGC-------ACuCCGCGACGAG--CUa--CGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 9381 | 0.73 | 0.108087 |
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Target: 5'- -uGCCGUcGGGUGCcugcuggcGCUUGAUGCGCu -3' miRNA: 3'- auCGGCAcUCCGCGa-------CGAGCUACGCG- -5' |
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| 1256 | 5' | -58.8 | NC_001317.1 | + | 1832 | 1.09 | 0.000159 |
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Target: 5'- uUAGCCGUGAGGCGCUGCUCGAUGCGCu -3' miRNA: 3'- -AUCGGCACUCCGCGACGAGCUACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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