Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12560 | 5' | -52.2 | NC_003345.1 | + | 63404 | 0.66 | 0.912209 |
Target: 5'- ---aCCGCGUaGACCGGGUAa--GCu -3' miRNA: 3'- uauaGGCGUAaCUGGCCCAUagaCGc -5' |
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12560 | 5' | -52.2 | NC_003345.1 | + | 43804 | 0.66 | 0.898103 |
Target: 5'- --uUCCGCAaucacgaUUG-CCGGGUAguucgUUGCGu -3' miRNA: 3'- uauAGGCGU-------AACuGGCCCAUa----GACGC- -5' |
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12560 | 5' | -52.2 | NC_003345.1 | + | 44154 | 0.68 | 0.825725 |
Target: 5'- cUAUCCGCAUUGACCGGa-------- -3' miRNA: 3'- uAUAGGCGUAACUGGCCcauagacgc -5' |
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12560 | 5' | -52.2 | NC_003345.1 | + | 44922 | 1.08 | 0.003434 |
Target: 5'- aAUAUCCGCAUUGACCGGGUAUCUGCGa -3' miRNA: 3'- -UAUAGGCGUAACUGGCCCAUAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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