Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12562 | 3' | -51.2 | NC_003345.1 | + | 55712 | 0.67 | 0.931717 |
Target: 5'- aCUCGUcCGGCGguggUGGGGcgGGUGaaCCCGg -3' miRNA: 3'- -GAGCAaGCUGCa---ACUCC--UCAUa-GGGC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 54571 | 0.68 | 0.920309 |
Target: 5'- gCUCuUUCGACGgcgauacUGAGGAGgaaAUCCuCGa -3' miRNA: 3'- -GAGcAAGCUGCa------ACUCCUCa--UAGG-GC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 13362 | 0.68 | 0.907818 |
Target: 5'- cCUUGUUCGugauacagcuCGUaGA-GAGUAUCCCGg -3' miRNA: 3'- -GAGCAAGCu---------GCAaCUcCUCAUAGGGC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 2486 | 0.68 | 0.901172 |
Target: 5'- ---cUUCGAgGUc-GGGAGUGUCCCGa -3' miRNA: 3'- gagcAAGCUgCAacUCCUCAUAGGGC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 71850 | 0.68 | 0.89426 |
Target: 5'- cCUCGcUgGACGaguUUGAcGGAGUGguUCCCGa -3' miRNA: 3'- -GAGCaAgCUGC---AACU-CCUCAU--AGGGC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 71576 | 0.7 | 0.838909 |
Target: 5'- aCUgGUguagagUCGGCGggUGGGGAGgucaaGUCCCGa -3' miRNA: 3'- -GAgCA------AGCUGCa-ACUCCUCa----UAGGGC- -5' |
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12562 | 3' | -51.2 | NC_003345.1 | + | 46652 | 1.11 | 0.003717 |
Target: 5'- aCUCGUUCGACGUUGAGGAGUAUCCCGa -3' miRNA: 3'- -GAGCAAGCUGCAACUCCUCAUAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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