Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12567 | 5' | -55.5 | NC_003345.1 | + | 50421 | 0.66 | 0.755011 |
Target: 5'- gGUGgaGCCGcuGGCGGAgauGGCGGUgGUGa -3' miRNA: 3'- -UAC--UGGU--UCGCCUac-UCGCCAgCACa -5' |
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12567 | 5' | -55.5 | NC_003345.1 | + | 53863 | 0.68 | 0.627424 |
Target: 5'- -cGGCCAAcucCGG-UGAGCuGGUCGUGa -3' miRNA: 3'- uaCUGGUUc--GCCuACUCG-CCAGCACa -5' |
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12567 | 5' | -55.5 | NC_003345.1 | + | 20136 | 0.69 | 0.570204 |
Target: 5'- cGUGGCaaguccguccccggCGAGCGGAUGAGUGGUaCGg-- -3' miRNA: 3'- -UACUG--------------GUUCGCCUACUCGCCA-GCaca -5' |
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12567 | 5' | -55.5 | NC_003345.1 | + | 60442 | 1.05 | 0.002551 |
Target: 5'- aAUGACCAAGCGGAUGAGCGGUCGUGUc -3' miRNA: 3'- -UACUGGUUCGCCUACUCGCCAGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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