miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12569 5' -53.3 NC_003345.1 + 68503 0.66 0.862348
Target:  5'- --aCCCGCucGCCGCcguccACUUCGAGGg -3'
miRNA:   3'- uugGGGCGuuCGGCGc----UGAAGUUUUa -5'
12569 5' -53.3 NC_003345.1 + 12260 0.66 0.845299
Target:  5'- gAACCCUGCuccggugggccgGAGCCGaugaaCGACUcaUCGAAGUg -3'
miRNA:   3'- -UUGGGGCG------------UUCGGC-----GCUGA--AGUUUUA- -5'
12569 5' -53.3 NC_003345.1 + 27753 0.67 0.808518
Target:  5'- cAACCUCGCAAGCaacuuGCGACgaUGAAAg -3'
miRNA:   3'- -UUGGGGCGUUCGg----CGCUGaaGUUUUa -5'
12569 5' -53.3 NC_003345.1 + 38437 0.69 0.649566
Target:  5'- --aCCCGUAGGCuuCGCGGcCUUCGAGGUa -3'
miRNA:   3'- uugGGGCGUUCG--GCGCU-GAAGUUUUA- -5'
12569 5' -53.3 NC_003345.1 + 21005 0.73 0.465283
Target:  5'- cAGCggCGCGAGCgGCGACUUCGAGGa -3'
miRNA:   3'- -UUGggGCGUUCGgCGCUGAAGUUUUa -5'
12569 5' -53.3 NC_003345.1 + 21771 0.74 0.396309
Target:  5'- -gUCCCGCAGGCCGCcgaccagauuaucGACUUCAu--- -3'
miRNA:   3'- uuGGGGCGUUCGGCG-------------CUGAAGUuuua -5'
12569 5' -53.3 NC_003345.1 + 14695 0.75 0.361331
Target:  5'- cGACCuuCCGCAuAGCCGCaGGCUUCGAGGa -3'
miRNA:   3'- -UUGG--GGCGU-UCGGCG-CUGAAGUUUUa -5'
12569 5' -53.3 NC_003345.1 + 63288 1.03 0.004721
Target:  5'- cAACCCCGCAAGCCGCGACUUCAAAAUc -3'
miRNA:   3'- -UUGGGGCGUUCGGCGCUGAAGUUUUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.