Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1257 | 3' | -56.2 | NC_001317.1 | + | 9918 | 0.66 | 0.494069 |
Target: 5'- gUGCUGCuGCUGGcaAUCGGUGGCGAGc -3' miRNA: 3'- gGCGAUGuCGGUC--UGGCCGUUGCUCc -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 17806 | 0.67 | 0.462837 |
Target: 5'- aCCGCUGCguuuaaAGCCucGGA-CGGUAAaacCGAGGa -3' miRNA: 3'- -GGCGAUG------UCGG--UCUgGCCGUU---GCUCC- -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 5569 | 0.67 | 0.483548 |
Target: 5'- -aGCuUACGGCCAacgaUGGCAACGAGu -3' miRNA: 3'- ggCG-AUGUCGGUcug-GCCGUUGCUCc -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 9295 | 0.69 | 0.341035 |
Target: 5'- gCCGCcGguGaCAGGCUGGCAaucagaccgGCGAGGc -3' miRNA: 3'- -GGCGaUguCgGUCUGGCCGU---------UGCUCC- -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 5982 | 0.69 | 0.355584 |
Target: 5'- cCCGCUGCgcggcuugagaGGCCgAGACCcGCAucaugugacgcuguGCGGGGc -3' miRNA: 3'- -GGCGAUG-----------UCGG-UCUGGcCGU--------------UGCUCC- -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 17024 | 0.7 | 0.308522 |
Target: 5'- cCUGCUGCGGCCAuguuACCGGCAAg---- -3' miRNA: 3'- -GGCGAUGUCGGUc---UGGCCGUUgcucc -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 4377 | 0.71 | 0.250058 |
Target: 5'- gCGCUGCAGCggguugugcuucgCAGugCGGCAGaauuCGAGa -3' miRNA: 3'- gGCGAUGUCG-------------GUCugGCCGUU----GCUCc -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 316 | 0.71 | 0.280603 |
Target: 5'- gCCGCUGCAGgacagguUCAGggagguaaacgacugGCuCGGCAugGAGGu -3' miRNA: 3'- -GGCGAUGUC-------GGUC---------------UG-GCCGUugCUCC- -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 9544 | 0.74 | 0.176113 |
Target: 5'- aCCGCaACAGCCGGGauGuGCAguACGAGGc -3' miRNA: 3'- -GGCGaUGUCGGUCUggC-CGU--UGCUCC- -5' |
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1257 | 3' | -56.2 | NC_001317.1 | + | 3465 | 1.12 | 0.000273 |
Target: 5'- aCCGCUACAGCCAGACCGGCAACGAGGc -3' miRNA: 3'- -GGCGAUGUCGGUCUGGCCGUUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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