Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1257 | 5' | -54.3 | NC_001317.1 | + | 3430 | 1.09 | 0.000543 |
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Target: 5'- aACCCCAACGUCGCCAACCGCAACAAGg -3' miRNA: 3'- -UGGGGUUGCAGCGGUUGGCGUUGUUC- -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 2154 | 0.8 | 0.082869 |
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Target: 5'- uGCUCCGGCGUCGUacuccagaCAGCCGCGGCGAa -3' miRNA: 3'- -UGGGGUUGCAGCG--------GUUGGCGUUGUUc -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 2124 | 0.76 | 0.137353 |
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Target: 5'- cGCCaCCuACGagGCCAGCCGCAcCGAGg -3' miRNA: 3'- -UGG-GGuUGCagCGGUUGGCGUuGUUC- -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 9350 | 0.72 | 0.284689 |
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Target: 5'- cACCCCGuaugccgcACGaaagCGCCAGCCGguACGGa -3' miRNA: 3'- -UGGGGU--------UGCa---GCGGUUGGCguUGUUc -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 19855 | 0.71 | 0.32441 |
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Target: 5'- -aCCCGAC-UgGCUGACCGCAAUggGg -3' miRNA: 3'- ugGGGUUGcAgCGGUUGGCGUUGuuC- -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 9525 | 0.69 | 0.415685 |
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Target: 5'- gGCCUCAAagacCGCCcaAACCGCAACAGc -3' miRNA: 3'- -UGGGGUUgca-GCGG--UUGGCGUUGUUc -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 18179 | 0.68 | 0.445977 |
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Target: 5'- -aCCCAGCa-CGCCAGCaGCAGCGAu -3' miRNA: 3'- ugGGGUUGcaGCGGUUGgCGUUGUUc -5' |
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| 1257 | 5' | -54.3 | NC_001317.1 | + | 16010 | 0.66 | 0.612133 |
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Target: 5'- aACCCgAAUGUCGUgAcCCuCGACAAGc -3' miRNA: 3'- -UGGGgUUGCAGCGgUuGGcGUUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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