Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12570 | 3' | -55.8 | NC_003345.1 | + | 7954 | 0.66 | 0.781905 |
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Target: 5'- cUCCGCaaucugaCUC-CC-CUGAUACGGGCAg -3' miRNA: 3'- -AGGUGa------GAGcGGaGAUUGUGCCCGUg -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 33376 | 0.66 | 0.752108 |
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Target: 5'- -gCACUC-CGCCUCaaggAACACGcuGGCGa -3' miRNA: 3'- agGUGAGaGCGGAGa---UUGUGC--CCGUg -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 67085 | 0.66 | 0.746012 |
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Target: 5'- aCCgGCUCUCGCUgauuguuuCGGGCGCu -3' miRNA: 3'- aGG-UGAGAGCGGagauugu-GCCCGUG- -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 34023 | 0.67 | 0.73164 |
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Target: 5'- gCCGugUUCGCCuUCUuACACGGGUAg -3' miRNA: 3'- aGGUgaGAGCGG-AGAuUGUGCCCGUg -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 76232 | 0.68 | 0.668286 |
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Target: 5'- aCUGCUCUaaccgaguagccCGCCUCgaugAugGUGGGCACg -3' miRNA: 3'- aGGUGAGA------------GCGGAGa---UugUGCCCGUG- -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 60749 | 0.68 | 0.646798 |
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Target: 5'- aCCAC-CUCGCgUUcgGGCGCGGGgAUg -3' miRNA: 3'- aGGUGaGAGCGgAGa-UUGUGCCCgUG- -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 63189 | 0.69 | 0.571661 |
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Target: 5'- cUCgGCUCUCGCUgaggguGCugGuGGCGCa -3' miRNA: 3'- -AGgUGAGAGCGGagau--UGugC-CCGUG- -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 46911 | 0.7 | 0.540005 |
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Target: 5'- cCCGCgugaCGCCUUUGACGacggcgaGGGCGCg -3' miRNA: 3'- aGGUGaga-GCGGAGAUUGUg------CCCGUG- -5' |
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| 12570 | 3' | -55.8 | NC_003345.1 | + | 63444 | 1.1 | 0.001007 |
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Target: 5'- cUCCACUCUCGCCUCUAACACGGGCACg -3' miRNA: 3'- -AGGUGAGAGCGGAGAUUGUGCCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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