Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12570 | 5' | -60.9 | NC_003345.1 | + | 69233 | 0.66 | 0.555258 |
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Target: 5'- uGGGaGaaGcUGAGCGAGGGCGaguUCCc -3' miRNA: 3'- uCCC-CggCcACUCGCUCCUGCgu-AGG- -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 17249 | 0.66 | 0.545181 |
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Target: 5'- uAGuGGGCCGGUauGAaaGAGGACGaCGUa- -3' miRNA: 3'- -UC-CCCGGCCA--CUcgCUCCUGC-GUAgg -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 1105 | 0.67 | 0.448548 |
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Target: 5'- uAGGGGCCGG--GGCGgaaaacAGGGgGCggCCc -3' miRNA: 3'- -UCCCCGGCCacUCGC------UCCUgCGuaGG- -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 45459 | 0.67 | 0.448548 |
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Target: 5'- -aGGGCgaGGUGAGCGAGaaggcGGCGguUCa -3' miRNA: 3'- ucCCCGg-CCACUCGCUC-----CUGCguAGg -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 467 | 0.68 | 0.42142 |
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Target: 5'- gGGGGGCCGGaGAGaCGGacGGACGgGg-- -3' miRNA: 3'- -UCCCCGGCCaCUC-GCU--CCUGCgUagg -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 16340 | 0.68 | 0.394452 |
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Target: 5'- gAGGGaGCUGGaacGAGCGgaugauuGGGAUGC-UCCg -3' miRNA: 3'- -UCCC-CGGCCa--CUCGC-------UCCUGCGuAGG- -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 49284 | 0.74 | 0.176244 |
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Target: 5'- gAGGGGUC-GUGgguucuucuaAGCGAGGACGC-UCCc -3' miRNA: 3'- -UCCCCGGcCAC----------UCGCUCCUGCGuAGG- -5' |
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| 12570 | 5' | -60.9 | NC_003345.1 | + | 63410 | 1.11 | 0.000315 |
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Target: 5'- gAGGGGCCGGUGAGCGAGGACGCAUCCg -3' miRNA: 3'- -UCCCCGGCCACUCGCUCCUGCGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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