Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12571 | 3' | -57.4 | NC_003345.1 | + | 53790 | 0.66 | 0.722965 |
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Target: 5'- gGGGAUGGauuCGACccucguGGCgGGGGUGAa -3' miRNA: 3'- -UCCUGCCgauGCUG------CCGgUUCCACUc -5' |
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| 12571 | 3' | -57.4 | NC_003345.1 | + | 67290 | 0.66 | 0.712688 |
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Target: 5'- uGGGCGGUUuugaccuCGGCGGCaacgaaagCGAcGGUGGGa -3' miRNA: 3'- uCCUGCCGAu------GCUGCCG--------GUU-CCACUC- -5' |
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| 12571 | 3' | -57.4 | NC_003345.1 | + | 41368 | 0.66 | 0.702337 |
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Target: 5'- uGGGACGGgUuacucaauCGuuaGGCCGAGGUGc- -3' miRNA: 3'- -UCCUGCCgAu-------GCug-CCGGUUCCACuc -5' |
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| 12571 | 3' | -57.4 | NC_003345.1 | + | 49925 | 0.66 | 0.670944 |
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Target: 5'- ----gGGCUACGACGGCCcGGuUGAc -3' miRNA: 3'- uccugCCGAUGCUGCCGGuUCcACUc -5' |
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| 12571 | 3' | -57.4 | NC_003345.1 | + | 50125 | 0.75 | 0.237533 |
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Target: 5'- -cGGCGGCUcCGGCGGUgGAGGUGGc -3' miRNA: 3'- ucCUGCCGAuGCUGCCGgUUCCACUc -5' |
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| 12571 | 3' | -57.4 | NC_003345.1 | + | 65705 | 0.98 | 0.005877 |
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Target: 5'- aAGGACGGCUA-GACGGCCAAGGUGAGg -3' miRNA: 3'- -UCCUGCCGAUgCUGCCGGUUCCACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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