Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12574 | 3' | -56 | NC_003345.1 | + | 60055 | 0.66 | 0.775998 |
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Target: 5'- gAGCgaaaGUCugCAUcgagGGCUGGAACGu- -3' miRNA: 3'- -UCGaag-CAGugGUG----CCGACCUUGCcc -5' |
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| 12574 | 3' | -56 | NC_003345.1 | + | 24571 | 0.66 | 0.766255 |
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Target: 5'- gAGCggUCGUCcuCgGCGGCaGGAcgcCGGGa -3' miRNA: 3'- -UCGa-AGCAGu-GgUGCCGaCCUu--GCCC- -5' |
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| 12574 | 3' | -56 | NC_003345.1 | + | 68575 | 0.66 | 0.766255 |
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Target: 5'- ---cUCGUCGCCACGGa-GGuACGGc -3' miRNA: 3'- ucgaAGCAGUGGUGCCgaCCuUGCCc -5' |
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| 12574 | 3' | -56 | NC_003345.1 | + | 55711 | 0.68 | 0.684554 |
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Target: 5'- ---cUCGUC-CgGCGGUggUGGGGCGGGu -3' miRNA: 3'- ucgaAGCAGuGgUGCCG--ACCUUGCCC- -5' |
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| 12574 | 3' | -56 | NC_003345.1 | + | 43126 | 0.68 | 0.66346 |
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Target: 5'- -aCUUCGUCuucaucgaccuCCACGG--GGAGCGGGa -3' miRNA: 3'- ucGAAGCAGu----------GGUGCCgaCCUUGCCC- -5' |
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| 12574 | 3' | -56 | NC_003345.1 | + | 68854 | 1.1 | 0.001304 |
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Target: 5'- gAGCUUCGUCACCACGGCUGGAACGGGc -3' miRNA: 3'- -UCGAAGCAGUGGUGCCGACCUUGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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