miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1258 3' -54.7 NC_001317.1 + 2743 0.73 0.209821
Target:  5'- uCGUcGCAGAGCUGGccaccGGCgAGCGCG-CGg -3'
miRNA:   3'- -GCA-CGUCUCGACCu----UUG-UCGCGCuGC- -5'
1258 3' -54.7 NC_001317.1 + 3639 1.1 0.000428
Target:  5'- cCGUGCAGAGCUGGAAACAGCGCGACGg -3'
miRNA:   3'- -GCACGUCUCGACCUUUGUCGCGCUGC- -5'
1258 3' -54.7 NC_001317.1 + 3833 0.66 0.56259
Target:  5'- gCGUGUccuGAGuCUGGAAACuGGCgGUGACc -3'
miRNA:   3'- -GCACGu--CUC-GACCUUUG-UCG-CGCUGc -5'
1258 3' -54.7 NC_001317.1 + 9802 0.66 0.573976
Target:  5'- cCGUGCAgGGGCUGuGccgcguacaAAGCGGCGgGAUc -3'
miRNA:   3'- -GCACGU-CUCGAC-C---------UUUGUCGCgCUGc -5'
1258 3' -54.7 NC_001317.1 + 17598 0.7 0.339277
Target:  5'- -cUGCAGgcGGCUGaGAAUGGCGCGGCu -3'
miRNA:   3'- gcACGUC--UCGACcUUUGUCGCGCUGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.