Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12582 | 3' | -56.5 | NC_003345.1 | + | 52388 | 0.66 | 0.784273 |
Target: 5'- uUCCGGGguGUUCccCUgaaCC-GUCUCGGCg -3' miRNA: 3'- -AGGCCCauCAAGa-GA---GGcCAGAGCCG- -5' |
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12582 | 3' | -56.5 | NC_003345.1 | + | 38077 | 0.71 | 0.477632 |
Target: 5'- -gUGGGUGGUcguUCaCUCCGGcUCUCGGa -3' miRNA: 3'- agGCCCAUCA---AGaGAGGCC-AGAGCCg -5' |
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12582 | 3' | -56.5 | NC_003345.1 | + | 22011 | 0.66 | 0.754241 |
Target: 5'- aCCGaGGUAGagagcCUacaUCCGGuucucaaUCUCGGCa -3' miRNA: 3'- aGGC-CCAUCaa---GAg--AGGCC-------AGAGCCG- -5' |
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12582 | 3' | -56.5 | NC_003345.1 | + | 18693 | 0.66 | 0.745304 |
Target: 5'- uUCCauuucGUAGauUUCUCUCUGGUCUguuugaCGGCa -3' miRNA: 3'- -AGGcc---CAUC--AAGAGAGGCCAGA------GCCG- -5' |
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12582 | 3' | -56.5 | NC_003345.1 | + | 12214 | 0.69 | 0.61979 |
Target: 5'- uUCCGGGUuGUUCcaaugCUCCGGggguucgUCUCGa- -3' miRNA: 3'- -AGGCCCAuCAAGa----GAGGCC-------AGAGCcg -5' |
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12582 | 3' | -56.5 | NC_003345.1 | + | 795 | 0.99 | 0.007001 |
Target: 5'- cUCCGGGUAGUUCUCUCCGGUUcCGGCc -3' miRNA: 3'- -AGGCCCAUCAAGAGAGGCCAGaGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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