miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12588 5' -50.6 NC_003345.1 + 63921 0.69 0.895967
Target:  5'- uCGGAGUCG-UCUCGAAcaacGAGGUCGa -3'
miRNA:   3'- -GCUUCGGCuGGAGCUUaac-UUCCAGC- -5'
12588 5' -50.6 NC_003345.1 + 14573 0.69 0.8866
Target:  5'- aCGGAGCgGcaaucuuCCUCGAAgccugcggcuaugcgGAAGGUCGa -3'
miRNA:   3'- -GCUUCGgCu------GGAGCUUaa-------------CUUCCAGC- -5'
12588 5' -50.6 NC_003345.1 + 4580 0.7 0.849176
Target:  5'- uCGAGGUCGGCUUCGcuu---AGGUCGa -3'
miRNA:   3'- -GCUUCGGCUGGAGCuuaacuUCCAGC- -5'
12588 5' -50.6 NC_003345.1 + 1805 0.72 0.743792
Target:  5'- gGAAGCU-ACCUCGAAUUGAcgAGGaUUGa -3'
miRNA:   3'- gCUUCGGcUGGAGCUUAACU--UCC-AGC- -5'
12588 5' -50.6 NC_003345.1 + 28595 0.73 0.701315
Target:  5'- aGAAGCCGAggcgcugaaCCUCuGGUUGccGGUCGg -3'
miRNA:   3'- gCUUCGGCU---------GGAGcUUAACuuCCAGC- -5'
12588 5' -50.6 NC_003345.1 + 60661 0.74 0.646787
Target:  5'- gGGuaguGCCGACCUCGAcUUuGAGGUUGa -3'
miRNA:   3'- gCUu---CGGCUGGAGCUuAAcUUCCAGC- -5'
12588 5' -50.6 NC_003345.1 + 4488 1.11 0.004008
Target:  5'- gCGAAGCCGACCUCGAAUUGAAGGUCGg -3'
miRNA:   3'- -GCUUCGGCUGGAGCUUAACUUCCAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.