miRNA: 3'- -UCCUUUcCAGCUGUc-UGAGUGGCCGU- -5'
miRNA: 3'- uCCu-UUCCAGC-UGUCUgA-----GUGGCCGU- -5'
miRNA: 3'- -UCCUUUCCAGCUGUCUGAGUGGCCGU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1259 | 3' | -54.7 | NC_001317.1 | + | 19007 | 0.69 | 0.384221 |
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Target: 5'- gGGGAAAcGUCGAUAcaGCUUGCCGGUAc -3' miRNA: 3'- -UCCUUUcCAGCUGUc-UGAGUGGCCGU- -5' |
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| 1259 | 3' | -54.7 | NC_001317.1 | + | 2671 | 0.7 | 0.339277 |
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Target: 5'- uGGucGAGGUCGaACAGGgUgcagcCACCGGCAa -3' miRNA: 3'- uCCu-UUCCAGC-UGUCUgA-----GUGGCCGU- -5' |
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| 1259 | 3' | -54.7 | NC_001317.1 | + | 6358 | 1.08 | 0.000567 |
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Target: 5'- cAGGAAAGGUCGACAGACUCACCGGCAu -3' miRNA: 3'- -UCCUUUCCAGCUGUCUGAGUGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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