Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1259 | 5' | -56.6 | NC_001317.1 | + | 276 | 0.66 | 0.479601 |
Target: 5'- cCUGCaGCGGCGAcaggucguuacGcacaaauaccuuUGCCACUUUCUCa -3' miRNA: 3'- cGACG-CGCCGUU-----------C------------ACGGUGAAGGAGc -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 15899 | 0.66 | 0.479601 |
Target: 5'- aUUGCGCGGCG-GUG-CugUUCCa-- -3' miRNA: 3'- cGACGCGCCGUuCACgGugAAGGagc -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 14590 | 0.66 | 0.458703 |
Target: 5'- -aUGCGCGGCuuaacgccGGUGaCCGCUUCggCa -3' miRNA: 3'- cgACGCGCCGu-------UCAC-GGUGAAGgaGc -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 16099 | 0.66 | 0.45767 |
Target: 5'- cCUGCGCGGCGucagucuggcggcGGUGgCGCagUCCgaggUCGa -3' miRNA: 3'- cGACGCGCCGU-------------UCACgGUGa-AGG----AGC- -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 5980 | 0.66 | 0.428294 |
Target: 5'- cGCUGCGCGGCuuGAGagGCCGagaCC-CGc -3' miRNA: 3'- -CGACGCGCCG--UUCa-CGGUgaaGGaGC- -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 16226 | 0.67 | 0.389668 |
Target: 5'- cCUGCGUGGUGAGUGCCGgguaggUCaugCGg -3' miRNA: 3'- cGACGCGCCGUUCACGGUga----AGga-GC- -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 24636 | 0.7 | 0.25937 |
Target: 5'- gGCUuCGCGGCGcAGUagcucgGCCGCUUCUUUGc -3' miRNA: 3'- -CGAcGCGCCGU-UCA------CGGUGAAGGAGC- -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 2363 | 0.7 | 0.252532 |
Target: 5'- cCUGCGCGGCAAaguccaUGCC-CUUCCa-- -3' miRNA: 3'- cGACGCGCCGUUc-----ACGGuGAAGGagc -5' |
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1259 | 5' | -56.6 | NC_001317.1 | + | 6322 | 1.11 | 0.000208 |
Target: 5'- cGCUGCGCGGCAAGUGCCACUUCCUCGa -3' miRNA: 3'- -CGACGCGCCGUUCACGGUGAAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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