Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12590 | 3' | -51.3 | NC_003345.1 | + | 57197 | 0.66 | 0.951434 |
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Target: 5'- gGAAGCCCUCGUUGu--CGUcuuugcGGGc--- -3' miRNA: 3'- -CUUCGGGAGCAACuuuGCA------CCCuaca -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 49711 | 0.66 | 0.948722 |
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Target: 5'- -uGGCCgCUC-UUGAAaaggcagucuccuauAUGUGGGAUGUu -3' miRNA: 3'- cuUCGG-GAGcAACUU---------------UGCACCCUACA- -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 42438 | 0.66 | 0.931475 |
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Target: 5'- --uGCCCUCGau---GCGUGGGAa-- -3' miRNA: 3'- cuuCGGGAGCaacuuUGCACCCUaca -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 39771 | 0.67 | 0.91356 |
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Target: 5'- -uGGCCCUCGUucuucgUGGAcuCGUccuucGGGAUGUu -3' miRNA: 3'- cuUCGGGAGCA------ACUUu-GCA-----CCCUACA- -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 45611 | 0.67 | 0.907028 |
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Target: 5'- --uGCCCUCGaUGAaggccGACGUGGcGcgGUu -3' miRNA: 3'- cuuCGGGAGCaACU-----UUGCACC-CuaCA- -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 62809 | 0.67 | 0.900218 |
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Target: 5'- aGAGGCgCUUGUUGAuuGGCGUGGucGUGa -3' miRNA: 3'- -CUUCGgGAGCAACU--UUGCACCc-UACa -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 6888 | 0.68 | 0.893135 |
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Target: 5'- aGggGUUgUCGUcaGAAGCGUGGGgcGUc -3' miRNA: 3'- -CuuCGGgAGCAa-CUUUGCACCCuaCA- -5' |
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| 12590 | 3' | -51.3 | NC_003345.1 | + | 6739 | 1.08 | 0.004167 |
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Target: 5'- gGAAGCCCUCGUUGAAACGUGGGAUGUg -3' miRNA: 3'- -CUUCGGGAGCAACUUUGCACCCUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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