Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12590 | 5' | -55 | NC_003345.1 | + | 10386 | 0.66 | 0.813421 |
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Target: 5'- cGACGCCCCACGgUgg-GGCGuuuCGc- -3' miRNA: 3'- uCUGCGGGGUGCgAagaCUGCu--GUug -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 67378 | 0.66 | 0.804132 |
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Target: 5'- gGGGCGCUCgACGCUggUGuuccaGCGGCGAa -3' miRNA: 3'- -UCUGCGGGgUGCGAagAC-----UGCUGUUg -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 72404 | 0.66 | 0.79467 |
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Target: 5'- cGGugGCCCCGac-----GACGACGACg -3' miRNA: 3'- -UCugCGGGGUgcgaagaCUGCUGUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 18012 | 0.67 | 0.775266 |
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Target: 5'- -cACGCCCUGauaacCGCUgaaggGGCGACGACa -3' miRNA: 3'- ucUGCGGGGU-----GCGAaga--CUGCUGUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 32295 | 0.67 | 0.734832 |
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Target: 5'- uGGACGCgCCGCcccGUgUUCUGACGuuCGACa -3' miRNA: 3'- -UCUGCGgGGUG---CG-AAGACUGCu-GUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 56938 | 0.68 | 0.703426 |
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Target: 5'- --uCGUCUUACuGCUUCUGACGACcuAACu -3' miRNA: 3'- ucuGCGGGGUG-CGAAGACUGCUG--UUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 30875 | 0.69 | 0.639083 |
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Target: 5'- aGGACGaccgaguugaCUCUGCGCUUCUaaaccGGCGGCGGCg -3' miRNA: 3'- -UCUGC----------GGGGUGCGAAGA-----CUGCUGUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 22738 | 0.69 | 0.639083 |
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Target: 5'- cGGACGCCUucguucuguuCGuCGCUUCUGACaacCAGCa -3' miRNA: 3'- -UCUGCGGG----------GU-GCGAAGACUGcu-GUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 46918 | 0.72 | 0.461306 |
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Target: 5'- aGGACaaCCCGCGUgacgccUUUGACGACGGCg -3' miRNA: 3'- -UCUGcgGGGUGCGa-----AGACUGCUGUUG- -5' |
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| 12590 | 5' | -55 | NC_003345.1 | + | 6782 | 1.09 | 0.00164 |
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Target: 5'- gAGACGCCCCACGCUUCUGACGACAACc -3' miRNA: 3'- -UCUGCGGGGUGCGAAGACUGCUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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