Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12591 | 3' | -55.6 | NC_003345.1 | + | 2135 | 0.66 | 0.826863 |
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Target: 5'- gGGCGGCGAGA-GUGaCAUCGCu--- -3' miRNA: 3'- gCCGCUGCUCUaCACaGUGGUGccug -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 68745 | 0.66 | 0.799689 |
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Target: 5'- uGGCGAcCGGGAUGcGcUCGCa--GGACg -3' miRNA: 3'- gCCGCU-GCUCUACaC-AGUGgugCCUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 34100 | 0.67 | 0.720757 |
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Target: 5'- -uGCGGCGA-AUGUcGUCgcuuucaccACCACGGACg -3' miRNA: 3'- gcCGCUGCUcUACA-CAG---------UGGUGCCUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 25429 | 0.68 | 0.668414 |
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Target: 5'- gGGCGGCGaAGAagacgugGUGauUCACguCACGGACg -3' miRNA: 3'- gCCGCUGC-UCUa------CAC--AGUG--GUGCCUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 68411 | 0.7 | 0.583468 |
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Target: 5'- aCGGCGGCGAGcgGguucGUCAUCGa-GACa -3' miRNA: 3'- -GCCGCUGCUCuaCa---CAGUGGUgcCUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 2617 | 0.7 | 0.572935 |
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Target: 5'- aCGGCGACGGcGGUaGUGaCACCGaaGACg -3' miRNA: 3'- -GCCGCUGCU-CUA-CACaGUGGUgcCUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 69739 | 0.72 | 0.45188 |
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Target: 5'- gCGGCGACGAcGAUgGUGUUACCAUcaACg -3' miRNA: 3'- -GCCGCUGCU-CUA-CACAGUGGUGccUG- -5' |
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| 12591 | 3' | -55.6 | NC_003345.1 | + | 7796 | 1.11 | 0.001195 |
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Target: 5'- aCGGCGACGAGAUGUGUCACCACGGACg -3' miRNA: 3'- -GCCGCUGCUCUACACAGUGGUGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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