Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12594 | 3' | -51.9 | NC_003345.1 | + | 63401 | 0.67 | 0.892275 |
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Target: 5'- gCGACcGC-GUAgaccGGGuaaGCUCCCUCCg -3' miRNA: 3'- -GCUGuUGaCAU----UCCugaCGAGGGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 11935 | 0.68 | 0.860794 |
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Target: 5'- cCGGCAACgGUcgccguaGAGGuCaaCUCCCUCCa -3' miRNA: 3'- -GCUGUUGaCA-------UUCCuGacGAGGGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 41981 | 0.68 | 0.853334 |
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Target: 5'- aGGuCAGCgaau-GGGCUGUUCuCCUCCa -3' miRNA: 3'- gCU-GUUGacauuCCUGACGAG-GGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 27095 | 0.69 | 0.836098 |
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Target: 5'- gGACGACgaGUA---ACUGCgCCCUCCa -3' miRNA: 3'- gCUGUUGa-CAUuccUGACGaGGGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 35325 | 0.69 | 0.836098 |
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Target: 5'- gGAgGAgUGUAAGGAgUGgUCgCCUCUc -3' miRNA: 3'- gCUgUUgACAUUCCUgACgAG-GGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 20213 | 0.69 | 0.80867 |
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Target: 5'- gGACGACgac---GAC-GCUCCCUCCg -3' miRNA: 3'- gCUGUUGacauucCUGaCGAGGGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 10948 | 0.73 | 0.586972 |
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Target: 5'- cCGGCGAUUGUucggcAGGACUcg-CCCUCCg -3' miRNA: 3'- -GCUGUUGACAu----UCCUGAcgaGGGAGG- -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 69570 | 0.77 | 0.394583 |
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Target: 5'- gCGGCGGC-GUGAcgcaGACUGCUCCCUCg -3' miRNA: 3'- -GCUGUUGaCAUUc---CUGACGAGGGAGg -5' |
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| 12594 | 3' | -51.9 | NC_003345.1 | + | 10806 | 1.12 | 0.002273 |
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Target: 5'- gCGACAACUGUAAGGACUGCUCCCUCCc -3' miRNA: 3'- -GCUGUUGACAUUCCUGACGAGGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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