Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12596 | 5' | -54.1 | NC_003345.1 | + | 5702 | 0.66 | 0.84363 |
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Target: 5'- -aGCUCCGAc-GUGGaagcaguCACGACGUUCGa -3' miRNA: 3'- caCGGGGCUaaCGCU-------GUGCUGCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 9790 | 0.68 | 0.750067 |
|
Target: 5'- -aGCCCCaccGAcaGCGACAgCGACGUUgAa -3' miRNA: 3'- caCGGGG---CUaaCGCUGU-GCUGCAAgU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 14122 | 1.09 | 0.0022 |
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Target: 5'- aGUGCCCCGAUUGCGACACGACGUUCAu -3' miRNA: 3'- -CACGGGGCUAACGCUGUGCUGCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 21209 | 0.69 | 0.697853 |
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Target: 5'- -gGUCuUCGGUUGgGAUACGugGUUCGc -3' miRNA: 3'- caCGG-GGCUAACgCUGUGCugCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 30289 | 0.66 | 0.844482 |
|
Target: 5'- aUGCCCCGA--GCGGCcuACcgugGugGUUCGc -3' miRNA: 3'- cACGGGGCUaaCGCUG--UG----CugCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 33297 | 0.73 | 0.475402 |
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Target: 5'- -cGUUCCGGUUGCGAaagcggccaACGACGUUUAc -3' miRNA: 3'- caCGGGGCUAACGCUg--------UGCUGCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 59776 | 0.66 | 0.861075 |
|
Target: 5'- -gGCCCCGGUUauUGACGCgGugGUUUg -3' miRNA: 3'- caCGGGGCUAAc-GCUGUG-CugCAAGu -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 60337 | 0.7 | 0.665697 |
|
Target: 5'- -gGCCCUGAUUGCGuu-CGcUGUUCAg -3' miRNA: 3'- caCGGGGCUAACGCuguGCuGCAAGU- -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 62554 | 0.69 | 0.718999 |
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Target: 5'- gGUGUCCCGAgu-CGAUGCGACGa--- -3' miRNA: 3'- -CACGGGGCUaacGCUGUGCUGCaagu -5' |
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| 12596 | 5' | -54.1 | NC_003345.1 | + | 71171 | 0.66 | 0.836731 |
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Target: 5'- -cGCCCCGAUaaucagggaauuacgGCGGCuACGAuuguCGUUCu -3' miRNA: 3'- caCGGGGCUAa--------------CGCUG-UGCU----GCAAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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