Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12597 | 5' | -52.9 | NC_003345.1 | + | 16395 | 0.66 | 0.887356 |
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Target: 5'- gGAAGGCGaAG-GGCGA-CUUCCUCa- -3' miRNA: 3'- -CUUCUGCcUCgCCGUUaGAAGGAGcu -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 5524 | 0.66 | 0.879906 |
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Target: 5'- ---uACGGAGCGGgGGUaucCCUCGGu -3' miRNA: 3'- cuucUGCCUCGCCgUUAgaaGGAGCU- -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 74450 | 0.67 | 0.864254 |
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Target: 5'- gGAGGuCGGGGCGGCca--UUCCaccuaUCGAg -3' miRNA: 3'- -CUUCuGCCUCGCCGuuagAAGG-----AGCU- -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 33504 | 0.69 | 0.772999 |
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Target: 5'- -uGGAUGGGcGCGGU--UCUUCCUCu- -3' miRNA: 3'- cuUCUGCCU-CGCCGuuAGAAGGAGcu -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 10837 | 0.69 | 0.772999 |
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Target: 5'- cGAGACGGAG-GGCGAg--UCCUgcCGAa -3' miRNA: 3'- cUUCUGCCUCgCCGUUagaAGGA--GCU- -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 58225 | 0.69 | 0.752685 |
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Target: 5'- uGAAcACGuAGCGGUAcgCUUCUUCGAg -3' miRNA: 3'- -CUUcUGCcUCGCCGUuaGAAGGAGCU- -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 47226 | 0.69 | 0.731884 |
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Target: 5'- gGAAGAUGaGAGCGGCcugCUgagccgUCUCGAa -3' miRNA: 3'- -CUUCUGC-CUCGCCGuuaGAa-----GGAGCU- -5' |
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| 12597 | 5' | -52.9 | NC_003345.1 | + | 14568 | 1.1 | 0.002317 |
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Target: 5'- cGAAGACGGAGCGGCAAUCUUCCUCGAa -3' miRNA: 3'- -CUUCUGCCUCGCCGUUAGAAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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