Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12598 | 5' | -55.1 | NC_003345.1 | + | 31428 | 0.66 | 0.828458 |
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Target: 5'- aCAAGCUGGACGUucGGCgCUGgUguuagCGCGu -3' miRNA: 3'- cGUUCGGCCUGUA--UCG-GAUgGa----GCGC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 13815 | 0.66 | 0.819505 |
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Target: 5'- aGCGGGCCGGugAgGGUa-ACUUCGUu -3' miRNA: 3'- -CGUUCGGCCugUaUCGgaUGGAGCGc -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 46085 | 0.66 | 0.801044 |
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Target: 5'- aGCGAGCCGGugAgaGGCCcacgucccCCUC-CGg -3' miRNA: 3'- -CGUUCGGCCugUa-UCGGau------GGAGcGC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 46493 | 0.66 | 0.801044 |
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Target: 5'- aCAAGCUggaGGACGUAGCCagcggcgACCUUuCGa -3' miRNA: 3'- cGUUCGG---CCUGUAUCGGa------UGGAGcGC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 29096 | 0.68 | 0.71079 |
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Target: 5'- aGCGGGCCGGugucuauguCGUAGUUcucgACCUCGgGc -3' miRNA: 3'- -CGUUCGGCCu--------GUAUCGGa---UGGAGCgC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 27169 | 0.68 | 0.678986 |
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Target: 5'- uGCGaaguAGCCGGAgu--GCUUGCCgUCGCGu -3' miRNA: 3'- -CGU----UCGGCCUguauCGGAUGG-AGCGC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 63639 | 0.68 | 0.668286 |
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Target: 5'- ---cGUCGGACA-GGCUgACCUCGUGa -3' miRNA: 3'- cguuCGGCCUGUaUCGGaUGGAGCGC- -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 59475 | 0.76 | 0.262321 |
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Target: 5'- uGCuGGUCGGugGUGG-CUACCUCGCu -3' miRNA: 3'- -CGuUCGGCCugUAUCgGAUGGAGCGc -5' |
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| 12598 | 5' | -55.1 | NC_003345.1 | + | 14695 | 1.1 | 0.001311 |
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Target: 5'- gGCAAGCCGGACAUAGCCUACCUCGCGu -3' miRNA: 3'- -CGUUCGGCCUGUAUCGGAUGGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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