Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 126 | 5' | -56.3 | AC_000006.1 | + | 18200 | 0.66 | 0.537605 |
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Target: 5'- cUGACUGGGAuuGGgaGCGcccuuggggGCAAgGCu -3' miRNA: 3'- uGCUGACCCU--CCgaCGCua-------CGUUgCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 22927 | 0.66 | 0.483753 |
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Target: 5'- cAUGGCuaccUGGGAggagaggaggauGGCUGCuGAaacaccUGCAGCGCc -3' miRNA: 3'- -UGCUG----ACCCU------------CCGACG-CU------ACGUUGCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 3687 | 0.67 | 0.462939 |
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Target: 5'- gGCGGCUGcGGcuGCcGCGGcUGCGGCGg -3' miRNA: 3'- -UGCUGAC-CCucCGaCGCU-ACGUUGCg -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 23505 | 0.67 | 0.452709 |
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Target: 5'- gACGGCgGGGuAGGCgaaguggGUGAgcuCAACGCu -3' miRNA: 3'- -UGCUGaCCC-UCCGa------CGCUac-GUUGCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 16473 | 0.67 | 0.432628 |
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Target: 5'- ---cCUGGGAGGCU-CGGUGacCGugGCg -3' miRNA: 3'- ugcuGACCCUCCGAcGCUAC--GUugCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 15554 | 0.68 | 0.375654 |
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Target: 5'- uGCGAgUccugccugcGGGGGGUgcagugGCGgcGCGGCGCg -3' miRNA: 3'- -UGCUgA---------CCCUCCGa-----CGCuaCGUUGCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 16624 | 0.7 | 0.285091 |
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Target: 5'- gGCGGC-GGGuGGCggUGCGGcgGCGGCGUu -3' miRNA: 3'- -UGCUGaCCCuCCG--ACGCUa-CGUUGCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 24167 | 0.73 | 0.190521 |
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Target: 5'- aGCGAuCUGGGAuGCUGCccgccaccUGCAACGCc -3' miRNA: 3'- -UGCU-GACCCUcCGACGcu------ACGUUGCG- -5' |
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| 126 | 5' | -56.3 | AC_000006.1 | + | 11824 | 1.12 | 0.000247 |
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Target: 5'- gACGACUGGGAGGCUGCGAUGCAACGCa -3' miRNA: 3'- -UGCUGACCCUCCGACGCUACGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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